Rapid Prototyping Three-Dimensional Cell/Gelatin/Fibrinogen Constructs for Medical Regeneration:
Rapid Prototyping Three-Dimensional Cell/Gelatin/Fibrinogen Constructs for Medical Regeneration:
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DOI：
10.1177/0883911507079451
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发表时间：
2007-07
影响因子：
1.7
通讯作者：
Wei Xu;X. Wang;Yongnian Yan;Weizhen Zheng;Z. Xiong;F. Lin;R. Wu;Renji Zhang
中科院分区：
文献类型：
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作者：
Wei Xu;X. Wang;Yongnian Yan;Weizhen Zheng;Z. Xiong;F. Lin;R. Wu;Renji Zhang
在再生医学中，需要快速制造具有明确结构和功能的组织或器官。通过自动快速成型技术成功构建了两种包含肝细胞、明胶和纤维蛋白原的细胞/基质结构，并用凝血酶稳定。在此过程中未发现明显的细胞损伤。力学表征表明，1:1比例的明胶/纤维蛋白混合物具有最大的弹性模量和抗压强度。显微镜和组织学观察表明，肝细胞包埋在明胶/纤维蛋白原基质中并增殖。免疫染色和生化分析显示包埋的肝细胞分泌白蛋白。纤维蛋白似乎是明胶基细胞组装基质的有利成分，因为它是生物可吸收的，易于操作，并支持体外细胞功能。
There is a need for rapid fabrication of tissue or organs with well-defined structures and functions in regenerative medicine. Two patterns of cell/matrix constructs containing hepatic cells, gelatin and fibrinogen were successfully created by automated rapid prototyping techniques and stabilized with thrombin. No apparent cell damage was found during the process. Mechanical characterization demonstrated that a 1:1 ratio gelatin/fibrin mixture had the greatest elasticity modulus and compressive strength. Microscopic and histological observations showed that hepatic cells were embedded in the gelatin/fibrinogen matrix and were proliferating. Immunostaining and biochemical analysis indicated that the embedded hepatocytes secreted albumin. Fibrin appears to be a favorable component for a gelatin based cell assembly matrix in that it is bioresorbable, easily manipulated, and supports in vitro cell functions.