INTEGRATIVE TRANSFORMATION OF THE MYCOTOXIN-PRODUCING FUNGUS, PENICILLIUM-PAXILLI
INTEGRATIVE TRANSFORMATION OF THE MYCOTOXIN-PRODUCING FUNGUS, PENICILLIUM-PAXILLI
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DOI：
10.1007/bf00351670
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发表时间：
1994-06-01
期刊：
影响因子：
2.5
通讯作者：
SCOTT, B
中科院分区：
文献类型：
--
作者：
ITOH, Y;JOHNSON, R;SCOTT, B
利用pAN7-1研制了paxilli青霉的高频转化系统。高达44%的初级转化体是异核体。在非选择性培养基上继代培养的原代转化子失去了对潮霉素的抗性，但这些原代转化子的单个孢子在选择性和非选择性培养基上都有丝分裂稳定。对生成的转化子的分子分析表明，78%的转化子具有单位点整合，其中一半包含一个单拷贝pAN7-1。凝胶电泳结果显示，paxilli至少有6条染色体，基因组总大小约为23.4 Mb。
A high frequency transformation system has been developed for Penicillium paxilli using pAN7-1. Up to 44% of the primary transformants were heterokaryons. Loss of hygromycin resistance was observed in primary transformants that were sub-cultured on non-selective media, but single spores of these primary transformants were mitotically stable on both selective and non-selective media. A molecular analysis of the transformants generated showed that 78% had single-site integrations, with half of these containing a single copy of pAN7-1. CHEF-gel electrophoresis showed that P. paxilli has at least six chromosomes with a total genome size of about 23.4 Mb.