Homologs of SCAR/WAVE complex components are required for epidermal cell morphogenesis in rice.
Homologs of SCAR/WAVE complex components are required for epidermal cell morphogenesis in rice.
复制标题
水稻表皮细胞形态发生需要 SCAR/WAVE 复合体成分的同系物。
DOI：
10.1093/jxb/erw214
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发表时间：
2016-07
影响因子：
6.9
通讯作者：
Hou S
中科院分区：
文献类型：
--
作者：
Zhou W;Wang Y;Wu Z;Luo L;Liu P;Yan L;Hou S
这项研究表明，水稻中的两种新蛋白-LPL 2和LPL 3-分别与SCAR/WAVE亚基PIR/SRA 1和NAP 1同源，通过肌动蛋白重组控制表皮细胞的形态发生。微丝肌动蛋白（F-肌动蛋白）在表皮细胞形态发生中起着重要作用。然而，有限数量的研究已经审查了肌动蛋白依赖的叶表皮细胞形态建成事件在水稻。在这项研究中，分离出两个隐性突变体：不太明显的叶表皮细胞2 -1（lpl 2 -1）和lpl 3 -1，其叶和茎表皮发育光滑的表面，具有较少的锯齿状铺路细胞（PC）裂片，并减少乳头。lpl 2 -1还表现出不规则的气孔模式，降低的植株高度，以及短的圆锥花序和根。分子遗传学研究表明，LPL 2和LPL 3分别编码PIROGI/Specifically Rac 1-associated protein 1（PIR/SRA 1）-like和NCK-associated protein 1（NAP 1）-like蛋白，这两种蛋白是cAMP受体抑制因子/Wiskott-Aldrich综合征蛋白家族verprolin同源蛋白（SCAR/WAVE）调节复合物的两个组分，其参与肌动蛋白成核和功能。在lpl 2和lpl 3扩展叶片中，表皮细胞表现出F-肌动蛋白的异常排列。此外，拟南芥pir的扭曲的毛状体可以部分恢复LPL 2的过表达。酵母双杂交试验表明，LPL 2可以直接与LPL 3在体外相互作用。总的来说，这些结果表明，LPL 2和LPL 3是两个功能保守的同源物的SCAR/WAVE复合物的组件，他们发挥了重要的作用，在控制水稻表皮细胞形态建成的组织F-肌动蛋白。
This study revealed that two novel proteins in rice – LPL2 and LPL3 – homologous to the SCAR/WAVE subunits PIR/SRA1 and NAP1, respectively, control epidermal cell morphogenesis through actin reorganization. Filamentous actins (F-actins) play a vital role in epidermal cell morphogenesis. However, a limited number of studies have examined actin-dependent leaf epidermal cell morphogenesis events in rice. In this study, two recessive mutants were isolated: less pronounced lobe epidermal cell2-1 (lpl2-1) and lpl3-1, whose leaf and stem epidermis developed a smooth surface, with fewer serrated pavement cell (PC) lobes, and decreased papillae. The lpl2-1 also exhibited irregular stomata patterns, reduced plant height, and short panicles and roots. Molecular genetic studies demonstrated that LPL2 and LPL3 encode the PIROGI/Specifically Rac1-associated protein 1 (PIR/SRA1)-like and NCK-associated protein 1 (NAP1)-like proteins, respectively, two components of the suppressor of cAMP receptor/Wiskott-Aldrich syndrome protein-family verprolin-homologous protein (SCAR/WAVE) regulatory complex involved in actin nucleation and function. Epidermal cells exhibited abnormal arrangement of F-actins in both lpl2 and lpl3 expanding leaves. Moreover, the distorted trichomes of Arabidopsis pir could be partially restored by an overexpression of LPL2. A yeast two-hybrid assay revealed that LPL2 can directly interact with LPL3 in vitro. Collectively, the results indicate that LPL2 and LPL3 are two functionally conserved homologs of the SCAR/WAVE complex components, and that they play an important role in controlling epidermal cell morphogenesis in rice by organising F-actin.