Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma
Clinical significance of LAMB3 and COL7A1 mRNA in esophageal squamous cell carcinoma
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DOI：
10.1016/j.ejso.2008.01.025
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发表时间：
2009-01-01
期刊：
影响因子：
3.8
通讯作者：
Mori, M.
中科院分区：
文献类型：
--
作者：
Kita, Y.;Mimori, K.;Mori, M.
目的：LAMB 3和COL 7A 1基因分别编码层粘连蛋白-5 β 3链和VII型胶原。它们构成基底膜区的主要成分。为探讨LAMB 3和COL 7A 1 mRNA在食管鳞状细胞癌（ESC）中的表达及其临床意义，采用实时荧光定量逆转录-聚合酶链反应（RT-PCR）方法检测LAMB 3和COL 7A 1 mRNA在食管癌组织（T）和相应正常组织（N）中的表达。LAMB 3和COL 7A 1表达的临床病理意义也被确定。结果：LAMB 3和COL 7A 1 mRNA在食管癌组织中的表达水平明显高于相应的正常食管组织。LAMB 3表达水平与浸润深度和静脉浸润显著相关（分别为p = 0.007和0.001）。COL 7A 1表达与肿瘤浸润深度和淋巴浸润显著相关（分别为p = 0.046、0.013）。结论：LAMB 3和COL 7A 1 mRNA的表达对预测ESC的恶性性质和预后有一定的价值。(c)2008爱思唯尔有限公司版权所有。
Aims: LAMB3 and COL7A1 genes code for the laminin-5 beta 3 chain and type VII collagen, respectively. They constitute the major components of the basement membrane zone. The aim of the current study was to clarify the clinical significance of LAMB3 and COL7A1 mRNA expression in esophageal squamous Cell carcinoma (ESC).Methods: We quantitated the expression of LAMB3 mRNA and COL7A1 mRNA in malignant esophageal tissues (T) and corresponding normal tissues (N) by real-time quantitative reverse transcription-polymerase chain reaction assays. The clinicopathologic significance of LAMB3 and COL7A1 expression was also determined. Paired T and N tissues were obtained from 66 patients who underwent curative esophagectomy.Results: The expression levels of LAMB3 and COL7A1 mRNAs were higher in malignant tissues than in the corresponding normal tissues. The level of LAMB3 expression was significantly correlated with the depth of invasion and venous invasion (p = 0.007 and 0.001, respectively). COL7A1 expression was significantly correlated with depth of tumor invasion and lymphatic invasion (p = 0.046, 0.013, respectively). The five-year survival rate was better in the 22 patients with relatively low expression of both LAMB3 and COL7A1 in comparison with the other 44 cases (p < 0.05).Conclusion: The evaluation of LAMB3 and COL7A1 mRNA expression is useful for predicting the malignant properties of ESC and may prove valuable in predicting the future Course of the disease. (c) 2008 Elsevier Ltd. All rights reserved.