PRODUCTION OF BOVINE TETRAPLOID EMBRYOS BY ELECTROFUSION AND THEIR DEVELOPMENTAL CAPABILITY INVITRO
PRODUCTION OF BOVINE TETRAPLOID EMBRYOS BY ELECTROFUSION AND THEIR DEVELOPMENTAL CAPABILITY INVITRO
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DOI：
10.1002/mrd.1120240303
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发表时间：
1989-11-01
期刊：
影响因子：
--
通讯作者：
NAKAHARA, T
中科院分区：
文献类型：
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作者：
IWASAKI, S;KONO, T;NAKAHARA, T
研究了两细胞牛胚卵裂球电融合制备四倍体胚的最佳条件。在电场强度为1.0 kV/cm、直流电脉冲为10或25 .mu的条件下，获得了较高的熔合率（73-95%）、活力和发育能力。SEC持续时间应用两次。细胞学研究表明，经电融合处理的胚胎中，有78.6%（11/14）为四倍体，其余为二倍体或六倍体。四倍体胚胎在体外具有发育至胚期的能力。
Optimal conditions of electrofusion for blastomeres of two-cell bovine embryos to produce tetraploid embryos were investigated. The high fusion rate (73-95%), viability, and developmental capacity were obtained under a field strength of 1.0 kV/cm with direct current pulses of 10 or 25 .mu.sec duration applied twice. Cytological study showed that 78.6% (11/14 embryos) of embryos exposed to electrofusion had tetraploid chromosome sets and the others were diploid or hexaploid. The tetraploid embryos had the capability to develop up to morulae stage in vitro.