In Vitro Experimental Study for the Determination of Cellular Axial Strain Threshold and Preferential Axial Strain from Cell Orientation Behavior in a Non-uniform Deformation Field
In Vitro Experimental Study for the Determination of Cellular Axial Strain Threshold and Preferential Axial Strain from Cell Orientation Behavior in a Non-uniform Deformation Field
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DOI：
10.1007/s12013-013-9643-3
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发表时间：
2013-05
影响因子：
2.6
通讯作者：
Y. Morita;S. Watanabe;Y. Ju;Shûhei Yamamoto
中科院分区：
文献类型：
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作者：
Y. Morita;S. Watanabe;Y. Ju;Shûhei Yamamoto
结缔组织内的细胞通常受到调节许多细胞行为的广泛的非均匀机械负荷。在本研究中，细胞取向角和膜的应变值之间的关系进行了全面的调查，使用非均匀的应变场。此外，细胞轴向应变阈值，这对应于发射细胞重定向反应，阐明。人骨髓间充质干细胞用于这些实验。在这项研究中，一个不均匀的应变分布很容易通过删除一个弹性室的一侧孔，在常用的单轴拉伸装置。使用数字图像相关方法在逐位置的基础上量化2D拉伸膜的应变。拉伸方向的法向应变在2.0 ~ 15.0%范围内连续变化。三维直方图的细胞频率，这是相关的细胞取向角和膜的法向应变，使它能够准确地确定轴向应变阈值。轴向应变阈值为4.4 ± 0.3%，与以往基于循环单轴拉伸刺激（均匀应变场）的研究相比是合理的。此外，还实现了细胞的优先轴向应变，这是首次引入的细胞特性，其值为-2.0 ± 0.1%。这项研究在三个方面是新颖的：（i）它精确而容易地确定了细胞的轴向应变阈值;（ii）它是第一个提出优先轴向应变的细胞;（iii）它系统地研究了细胞在非均匀应变场中的行为。
Cells within connective tissues are routinely subjected to a wide range of non-uniform mechanical loads that regulate many cell behaviors. In the present study, the relationship between cell orientation angle and strain value of the membrane was comprehensively investigated using an inhomogeneous strain field. Additionally, the cellular axial strain threshold, which corresponds to the launching of cell reorientation response, was elucidated. Human bone marrow mesenchymal stem cells were used for these experiments. In this study, an inhomogeneous strain distribution was easily created by removing one side holes of an elastic chamber in a commonly used uniaxial stretching device. The strains of 2D stretched membranes were quantified on a position-by-position basis using the digital image correlation method. The normal strain in the direction of stretch was changed continuously from 2.0 to 15.0 %. A 3D histogram of the cell frequency, which was correlated with the cell orientation angle and normal strain of the membrane, made it possible to determine the axial strain threshold accurately. The value of the axial strain threshold was 4.4 ± 0.3 %, which was reasonable compared with previous studies based on cyclic uniaxial stretch stimulation (homogeneous strain field). Additionally, preferential axial strain of cells, which was a cell property firstly introduced, was also achieved and the value was −2.0 ± 0.1 %. This study is novel in three respects: (i) it precisely and easily determined the axial strain threshold of cells; (ii) it is the first to suggest preferential axial strain of cells; and (iii) it methodically investigated cell behavior in an inhomogeneous strain field.