Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environments.
Natural variation in the extent of phosphorylation of bone phosphoproteins as a function of in vivo new bone formation induced by demineralized bone matrix in soft tissue and bony environments.
复制标题
骨磷蛋白磷酸化程度的自然变化是软组织和骨环境中脱矿骨基质诱导的体内新骨形成的函数。
DOI：
10.1042/bj20011272
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Fluckiger,Rudolf
中科院分区：
文献类型：
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作者：
Salih,Erdjan;Wang,Jinxi;Mah,James;Fluckiger,Rudolf
同种异体脱矿骨基质的植入物被放置在不同的体内环境中，即颅骨（骨）和皮下（软组织）部位。对生化成分进行了详细分析。每个种植体环境中骨桥蛋白 (OPN)、骨唾液蛋白 (BSP) 和磷酸钙 (Ca-P) 沉积的定量水平随着新骨形成的变化而变化，并且在颅骨和皮下部位的样本中存在显着差异。对此类植入物中亲和纯化的 OPN 和 BSP 的磷酸化程度进行定量表明：(i) 磷酸丝氨酸 (P-Ser) 的摩尔数/亲和纯化的 OPN 或 BSP 的摩尔数随植入时间和两个植入位点内骨形成的函数而变化，以及 (ii) 每个植入位点内总 OPN 和 BSP 提供的“有效 P-Ser 浓度”随时间变化并增加，约为 100%。颅骨中的 BSP 比皮下植入物高 5 倍。 P-Ser 摩尔数/BSP 摩尔数的峰值水平与颅骨植入物中 Ca-P 沉积的最大速率一致。颅骨和皮下植入物的 OPN 磷酸化水平也表明随着骨形成的变化而波动。因此，本研究首次提供了 OPN 和 BSP 磷酸化程度随矿化组织形成时间变化的自然变化的直接证据。对数据的进一步评估提供了第一个证据，证明颅骨植入物的 Ca-P 沉积速率与 P-Ser-BSP/P-Ser-OPN 比率之间存在直接和线性关系。皮下植入物的数据未能提供这种相关性。总体而言，目前的工作表明，生物矿化过程的自然生物进展遵循与解剖位置一致的严格标准。在软组织环境中，生物矿化无法以相同的方式进行。
Implants of allogenic demineralized bone matrix were placed in distinctin vivoenvironments, i.e. calvarial (bony) and subcutaneous (soft tissue) sites. Detailed analyses of the biochemical components were performed. Quantitative levels of osteopontin (OPN), bone sialoprotein (BSP) and calcium phosphate (Ca-P) deposition within each implant environment varied as a function of new bone formation, and were substantially different in samples from calvarial and subcutaneous sites. Quantification of the extent of phosphorylation of affinity-purified OPN and BSP from such implants indicated that: (i) the number of mols of phosphoserine (P-Ser)/mol of affinity-purified OPN or BSP varied as a function of implant time and bone formation within both implant sites, and (ii) the ‘effective P-Ser concentration’ provided by the total OPN and BSP within each implant site varied and increased as a function of time, being approx. 5-fold higher for BSP in calvarial compared with subcutaneous implants. Peak levels of mols of P-Ser/mol of BSP coincided with maximum rates of Ca-P deposition in calvarial implants. Levels of OPN phosphorylation from both calvarial and subcutaneous implants also indicated fluctuations as a function of bone formation. Hence the present study, for the first time, provides direct evidence of natural variation in the extent of phosphorylation of both OPN and BSP as a function of time of mineralized tissue formation. Further evaluation of the data provides the first evidence of a direct and linear relationship between the rate of Ca-P deposition and the ratio of P-Ser-BSP/P-Ser-OPN for calvarial implants. Data for subcutaneous implants failed to provide such correlation. Overall, the present work demonstrates that the natural biological progression of the process of biomineralization follows strict criteria consistent with the anatomical location. Biomineralization fails to proceed in the same way in a soft tissue environment.