AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana
AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana
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DOI：
10.1016/j.cub.2005.06.041
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发表时间：
2005-07-12
期刊：
影响因子：
9.2
通讯作者：
Campbell, MM
中科院分区：
文献类型：
--
作者：
Liang, YK;Dubos, C;Campbell, MM
气孔是植物叶片表面的动态气孔，控制植物的水分流失，调节光合作用对CO2的吸收[1]。气孔开度由气孔周围的两个保卫细胞控制[1]。当两个保卫细胞完全膨胀时，气孔张开，而膨压丧失导致气孔关闭。为了设置最合适的气孔开度为主导的环境条件下，保卫细胞响应多种内部和外部信号[2]。虽然对保卫细胞信号传导途径了解很多[2-9]，但对基因表达的变化如何参与气孔开度的控制知之甚少[10]。在这里，我们表明，AtMYB 61（At 1g 09540），基因编码的拟南芥R2 R3-MYB家族的转录因子的成员，是专门在保卫细胞中表达的方式与参与气孔开度的控制一致。功能获得和功能丧失突变体的分析表明，AtMYB 61的表达是足够的和必要的，从而减少气孔孔径气体交换的影响。总之，我们的数据提供的证据表明，AtMYB 61编码的第一个转录因子参与气孔关闭。
Stomata, dynamic pores found on the surfaces of plant leaves, control water loss from the plant and regulate the uptake of CO2 for photosynthesis [1]. Stomatal aperture is controlled by the two guard cells that surround the stomatal pore [1]. When the two guard cells are fully turgid, the pore gapes open, whereas turgor loss results in stomatal closure. In order to set the most appropriate stomatal aperture for the prevailing environmental conditions, guard cells respond to multiple internal and external signals [2]. Although much is known about guard-cell signaling pathways [2-9], rather little is known about how changes in gene expression are involved in the control of stomatal aperture [10]. We show here that AtMYB61 (At1g09540), a gene encoding a member of the Arabidopsis thaliana R2R3-MYB family of transcription factors, is specifically expressed in guard cells in a manner consistent with involvement in the control of stomatal aperture. Gain-of-function and loss-of-function mutant analyses reveal that AtMYB61 expression is both sufficient and necessary to bring about reductions in stomatal aperture with consequent effects on gas exchange. Taken together, our data provide evidence that AtMYB61 encodes the first transcription factor implicated in the closure of stomata.