Detection of deoxynivalenol contamination in wheat products in Thailand
Detection of deoxynivalenol contamination in wheat products in Thailand
复制标题
DOI：
10.4315/0362-028x-71.9.1931
复制
发表时间：
2008-09-01
影响因子：
2
通讯作者：
Kumagai, Susumu
中科院分区：
文献类型：
--
作者：
Poapolathep, Amnart;Poapolathep, Saranya;Kumagai, Susumu
于2007年2月至4月在泰国曼谷的超市共采集了三种小麦产品（30个面条、30个面包和30个谷物样品）的90个样品。采用高效液相色谱-紫外检测器检测小麦制品中脱氧雪腐镰刀菌烯醇（DON）的污染情况。使用多功能净化柱进行提取方法。定量限为0.10 mu 9中心点g（-1）从线性校准中获得的范围。调查发现，几乎94%的受DON污染的样品低于1 μ g中心点g（-1），这相当于美国食品和药物管理局的咨询水平。DON检出率为18.9%（90个样品中的17个），在面条和面包样品中分别有6.67%（30个样品中的2个）和16.67%（30个样品中的5个），水平分别为0.17 - 0.35和0.14 - 1.13 μ g中心点g（-1），而在33.33%（10/30）的谷物样品中，其水平为0.13至0.39 μ g中心点g（-1）。结果表明，从小麦制品，特别是面条，面包和谷物的消费中暴露于DON的风险水平非常低。
A total of ninety samples in three kinds of wheat products (30 noodle, 30 bread, and 30 cereal samples) were collected from the supermarkets in Bangkok, Thailand, from February to April 2007. The occurrence of deoxynivalenol (DON) contamination in wheat products was investigated using high-performance liquid chromatography equipped with a UV light detector. The extraction method was performed using a multifunctional cleanup column. The limit of quantification was 0.10 mu 9 center dot g(-1) from the range obtained in a linear calibration. The Survey found almost 94% of the DON-contaminated samples below 1 mu g center dot g(-1), which corresponds to the U.S. Food and Drug Administration advisory level. DON was detected in 18.9% (17 of 90) of all samples, in 6.67% (2 of 30) and 16.67% (5 of 30) of noodle and bread samples at levels from 0.17 to 0.35 and 0.14 to 1.13 mu g center dot g(-1), respectively, while it was in 33.33% (10 of 30) of cereal samples at levels from 0.13 to 0.39 mu g center dot g(-1). The results suggest that the exposure to DON from the consumption of wheat products, especially noodles, bread, and cereal, is at a very low risk level.