3D characterization of morphological changes in the intervertebral disc and endplate during aging: A propagation phase contrast synchrotron micro-tomography study.
3D characterization of morphological changes in the intervertebral disc and endplate during aging: A propagation phase contrast synchrotron micro-tomography study.
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DOI：
10.1038/srep43094
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发表时间：
2017-03-07
影响因子：
4.6
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Cao Y;Liao S;Zeng H;Ni S;Tintani F;Hao Y;Wang L;Wu T;Lu H;Duan C;Hu J
更好地了解椎间盘（IVD）的功能变化及其与终板的相互作用是阐明IVD退行性疾病（IDDD）发病机制的关键。到目前为止，同时描绘终板的3D微结构变化与老化和与IVD的相互作用仍然是一个技术挑战。我们的目的是使用PPCST来表征终板和IVD在老化过程中的3D形态学变化。首先通过PPCST评价从15天、4个月和24个月大的小鼠收获的腰椎4/5水平IVD，随后分析组织学切片以确认成像效率。老化后与年龄相关的趋势的定量评估，包括平均直径，体积分数和连接的运河，终板孔隙率和厚度，在4个月达到峰值，并在24个月显着下降。IVD体积在老化后始终表现出与终板相同的变化趋势。在本研究中，PPCST同时提供了终板中IVD和髓道网络的3D形态变化以及老化后相互作用的全面详细信息。结果表明，PPCST有潜力为更深入地了解IDDD的发病机制提供一个新的平台，并提供潜在的治疗靶点。
A better understanding of functional changes in the intervertebral disc (IVD) and interaction with endplate is essential to elucidate the pathogenesis of IVD degeneration disease (IDDD). To date, the simultaneous depiction of 3D micro-architectural changes of endplate with aging and interaction with IVD remains a technical challenge. We aim to characterize the 3D morphology changes of endplate and IVD during aging using PPCST. The lumbar vertebral level 4/5 IVDs harvested from 15-day-, 4- and 24-month-old mice were initially evaluated by PPCST with histological sections subsequently analyzed to confirm the imaging efficiency. Quantitative assessments of age-related trends after aging, including mean diameter, volume fraction and connectivity of the canals, and endplate porosity and thickness, reached a peak at 4 months and significantly decreased at 24 months. The IVD volume consistently exhibited same trend of variation with the endplate after aging. In this study, PPCST simultaneously provided comprehensive details of 3D morphological changes of the IVD and canal network in the endplate and the interaction after aging. The results suggest that PPCST has the potential to provide a new platform for attaining a deeper insight into the pathogenesis of IDDD, providing potential therapeutic targets.