Overexpression of vascular endothelial growth factor-C correlates with lymph node micrometastasis in submucosal esophageal cancer
Overexpression of vascular endothelial growth factor-C correlates with lymph node micrometastasis in submucosal esophageal cancer
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DOI：
10.1016/j.gassur.2006.01.009
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发表时间：
2006-07-01
影响因子：
3.2
通讯作者：
Aikou, Takashi
中科院分区：
文献类型：
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作者：
Matsumoto, Masataka;Natsugoe, Shoji;Aikou, Takashi
包括淋巴结微转移（LAW）在内的淋巴结转移是食管鳞状细胞癌（ESCC）最重要的预后因素之一。血管内皮生长因子C （VEGF-C）在淋巴管生成过程中起关键作用。我们检测了ESCC原发肿瘤的VEGF-C表达和肿瘤微血管密度，并分析了VEGF-C表达与粘膜下ESCC的临床病理表现（包括LAW）之间的关系。研究对象为87例粘膜下ESCC患者。对原发肿瘤进行VEGF-C和CD34免疫组化染色，对清扫淋巴结进行细胞角蛋白染色。通过CD34表达计算微血管密度，细胞角蛋白染色检测LMM。VEGF-C过表达与肿瘤浸润深度、淋巴浸润深度、淋巴结转移相关（P < 0.05、P < 0.0001、P < 0.0001）。微血管密度高与淋巴浸润和淋巴结转移相关（P < 0.005和P < 0.05）。细胞角蛋白染色8例及14例淋巴结检出LAW。与无淋巴结转移和无LAW的肿瘤相比，有淋巴结转移和/或LMM的肿瘤中VEGF-C过表达和微血管密度高（P < 0.0001和P < 0.01）。本研究结果表明，在粘膜下浸润的ESCC中，原发肿瘤VEGF-C过表达是包括LMM在内的淋巴结转移的高危因素。
Lymph node metastasis, including lymph node micrometastasis (LAW), is one of the most important prognostic factors in esophageal squamous cell carcinoma (ESCC). Vascular endothelial growth factor C (VEGF-C) plays a key role in the process of lymphangiogenesis. We examined VEGF-C expression and tumor inicrovessel density of the primary tumors in ESCC and analyzed relationships between VEGF-C expression and clinicopathologic findings including LAW in submucosal ESCC. The subjects were 87 patients with submucosal ESCC. Immunohistochemical staining of VEGF-C and CD34 was performed with primary tumors, and staining of cytokeratin was performed with dissected lymph nodes. Microvessel density was calculated from CD34 expression, and LMM was detected by cytokeratin staining. VEGF-C overexpression significantly correlated with depth of tumor invasion, lymphatic invasion, and lymph node metastasis (P < 0.05, P < 0.0001, and P < 0.0001, respectively). High microvessel density also correlated with lymphatic invasion and lymph node metastasis (P < 0.005 and P < 0.05, respectively). LAW was detected in 8 cases and 14 lymph nodes by cytokeratin staining. VEGF-C overexpression and high microvessel density were found in tumors with lymph node metastasis and/or LMM, compared with tumors without nodal metastasis or LAW (P < 0.0001 and P < 0.01, respectively). The present findings indicate that in ESCC with submucosal invasion, VEGF-C overexpression of the primary tumor is a strong high risk factor for lymph node metastasis, including LMM.