Characterization of AAT1: a gene involved in the regulation of amino acid transport in Saccharomyces cerevisiae.
Characterization of AAT1: a gene involved in the regulation of amino acid transport in Saccharomyces cerevisiae.
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AAT1 的表征：一种参与酿酒酵母氨基酸转运调节的基因。
DOI：
10.1099/00221287-135-9-2429
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Garrett,JM
中科院分区：
文献类型：
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作者：
Garrett,JM
已经鉴定出一类新的酿酒酵母突变体（aat1-氨基酸转运）。这些突变体无法在丰富培养基或补充了某些氨基酸（异亮氨酸、蛋氨酸、苯丙氨酸、酪氨酸或缬氨酸）的基本培养基上生长。这种表型与这些菌株中 leu2 等位基因的存在直接相关：aat1 LEU2 生物体在所有测试的培养基上正常生长。在不允许的培养基中预培养的aat1细胞中，通过亮氨酸特异性通透酶的亮氨酸摄取被抑制至野生型水平的<35%，并且在这些条件下一般氨基酸通透酶的活性也很低，因此aat1细胞无法在丰富的培养基上生长，因为它们不能摄取足够的亮氨酸来补充其营养缺陷型的需求。
A new class ofSaccharomyces cerevisiaemutants (aat1- amino acid transport) has been identified. These mutants are unable to grow on rich medium or on minimal medium supplemented with certain amino acids (isoleucine, methionine, phenylalanine, tyrosine or valine). This phenotype is directly linked to the presence of theleu2allele in these strains:aat1 LEU2organisms grow normally on all media tested. Leucine uptake through the leucine-specific permease is inhibited to <35% of wild-type levels inaat1cells preincubated in nonpermissive media, and the activity of the general amino acid permease is also low in these conditions,aat1cells are therefore unable to grow on rich media because they cannot take up enough leucine to supplement their auxotrophic requirement.