Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands
Increasing fibroblast response to materials using nanotopography: morphological and genetic measurements of cell response to 13-nm-high polymer demixed islands
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DOI：
10.1006/excr.2002.5498
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发表时间：
2002-05-15
影响因子：
3.7
通讯作者：
Curtsi, ASG
中科院分区：
文献类型：
--
作者：
Dalby, MJ;Yarwood, SJ;Curtsi, ASG
越来越清楚的是，细胞不仅可以响应微米尺度的地形，也可以响应纳米尺度的地形。可重复尺寸的纳米特征的生产严重依赖于昂贵且耗时的制造方法，例如电子束光刻。聚苯乙烯和聚苯乙烯的聚合物脱混已被发现可以产生可复制高度的纳米级孤岛，并且与平面表面相比，这些孤岛已被证明可以影响细胞的扩散。本研究观察了成纤维细胞对13纳米高岛屿反应的形态学、细胞骨架和分子变化。所采用的方法包括扫描电镜、荧光显微镜和1718基因芯片。结果表明，细胞通过广泛的基因上调来响应岛屿，特别是在细胞信号传导、增殖、细胞骨架和细胞外基质蛋白的产生方面。显微镜结果证实了微阵列的发现。(C) 2002 Elsevier Science （USA）。
It is becoming clear that cells can respond not only to micometric scale topography, but may also to nanometric scale topography. The production of reproducibly sized nanometric features has relied heavily on expensive and time-consuming methods of manufacture, such as electron beam lithography. Polymer demixing of polystyrene and polybromostyrene has been found to produce nanoscale islands of reproducible height, and the islands have been previously shown to effect cell spreading compared to planar surfaces. This study observes morphological, cytoskeletal, and molecular changes in fibroblast reaction to 13-nm-high islands. The methods employed include scanning electron microscopy, fluorescent microscopy, and 1718 gene microarray. The results show that the cells respond to the islands by broad gene up-regulation, notably in the areas of cell signaling, proliferation, cytoskeleton, and production of extracellular matrix proteins. Microscopical results provide confirmation of the microarray findings. (C) 2002 Elsevier Science (USA).