Characterization of the Primary Human Trophoblast Cell Secretome Using Stable Isotope Labeling With Amino Acids in Cell Culture.
Characterization of the Primary Human Trophoblast Cell Secretome Using Stable Isotope Labeling With Amino Acids in Cell Culture.
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DOI：
10.3389/fcell.2021.704781
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发表时间：
2021
影响因子：
5.5
通讯作者：
Jansson T
中科院分区：
文献类型：
--
作者：
Rosario FJ;Pardo S;Michelsen TM;Erickson K;Moore L;Powell TL;Weintraub ST;Jansson T
胎盘绒毛合体滋养层细胞是人体胎盘营养物质运输和激素分泌的上皮细胞，是胎儿发育和母体生理的重要调节因子。然而，原代人滋养层细胞(PHT)合成和分泌的蛋白质的特性尚不清楚。从正常足月胎盘绒毛组织(n=4/独立胎盘)分离的合胞化多核PHT细胞(n=4/独立胎盘)，采用细胞培养中氨基酸稳定同位素标记和条件培养液的质谱分析，鉴定其分泌的蛋白质及其相对合成速率。共鉴定出1,344种蛋白质，其中大多数以前从未报道过是由人类胎盘或滋养层细胞分泌的。大多数分泌的蛋白质参与能量和碳代谢、糖酵解、氨基酸的生物合成、嘌呤代谢和脂肪酸降解。组蛋白家族蛋白和线粒体蛋白是合成速度最慢的蛋白质，而与信号和质膜相关的蛋白质合成速度最快。在体内，PHT分泌组和已知的人类胎盘分泌到胎儿循环的蛋白质之间有显著的重叠。产生的数据将指导未来的实验，以确定个别分泌蛋白的功能，并将帮助我们更好地了解胎盘如何控制母婴生理。
The placental villus syncytiotrophoblast, the nutrient-transporting and hormone-producing epithelium of the human placenta, is a critical regulator of fetal development and maternal physiology. However, the identities of the proteins synthesized and secreted by primary human trophoblast (PHT) cells remain unknown. Stable Isotope Labeling with Amino Acids in Cell Culture followed by mass spectrometry analysis of the conditioned media was used to identify secreted proteins and obtain information about their relative rates of synthesis in syncytialized multinucleated PHT cells isolated from normal term placental villus tissue (n = 4/independent placenta). A total of 1,344 proteins were identified, most of which have not previously been reported to be secreted by the human placenta or trophoblast. The majority of secreted proteins are involved in energy and carbon metabolism, glycolysis, biosynthesis of amino acids, purine metabolism, and fatty acid degradation. Histone family proteins and mitochondrial proteins were among proteins with the slowest synthesis rate whereas proteins associated with signaling and the plasma membrane were synthesized rapidly. There was a significant overlap between the PHT secretome and proteins known be secreted to the fetal circulation by the human placenta in vivo. The generated data will guide future experiments to determine the function of individual secreted proteins and will help us better understand how the placenta controls maternal and fetal physiology.
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影响因子：
7.8
作者：
Dai DF;Karunadharma PP;Chiao YA;Basisty N;Crispin D;Hsieh EJ;Chen T;Gu H;Djukovic D;Raftery D;Beyer RP;MacCoss MJ;Rabinovitch PS
通讯作者：
Rabinovitch PS
影响因子：
4.8
作者：
Behura, Susanta K.;Kelleher, Andrew M.;Spencer, Thomas E.
通讯作者：
Spencer, Thomas E.
影响因子：
7.7
作者：
BRELJE, TC;PARSONS, JA;SORENSON, RL
通讯作者：
SORENSON, RL
DOI：
10.1074/mcp.m111.011429
发表时间：
2012-03
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Boisvert FM;Ahmad Y;Gierliński M;Charrière F;Lamont D;Scott M;Barton G;Lamond AI
通讯作者：
Lamond AI
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.