Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex
Capillary hydrophilic interaction chromatography/mass spectrometry for simultaneous determination of multiple neurotransmitters in primate cerebral cortex
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DOI：
10.1002/rcm.3251
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Logothetis, Nikos K.
中科院分区：
文献类型：
--
作者：
Zhang, Xiaozhe;Rauch, Alexander;Logothetis, Nikos K.
多种神经递质和神经调节剂控制和影响大脑功能。因此，对信号通路和行为背后的神经回路的深刻理解可能需要跟踪活性神经化学物质的浓度变化。在本研究中，我们证明了一种方法的可行性，该方法可以同时测定细胞外脑液（EBF）中六种神经递质的浓度：乙酰胆碱、血清素、多巴胺、γ-氨基丁酸（GABA）、谷氨酸和天冬氨酸。我们使用亲水相互作用色谱 (HILIC) 与串联质谱 (MS/MS) 结合来分析猴脑的 EBF。采用推拉采样方法以低 nL/min 的流速从有意识的猴子的前额皮质 (PFC) 收集 EBF。乙酰胆碱、血清素、多巴胺、GABA、谷氨酸和天冬氨酸的检测限分别为0.015、0.15、0.3、1.2、6和15飞摩尔，使我们能够从500 nL体内样品中同时定量测定这六种神经递质的浓度。我们得出的结论是，HILIC/MS/MS 与推拉采样方法相结合代表了一种同时监测 EBF 样本中神经递质的敏感技术。版权所有 (C) 2007 John Wiley & Sons, Ltd.
A diverse array of neurotransmitters and neuromodulators control and affect brain function. A profound understanding of the signaling pathways and the neural circuits underlying behavior is therefore likely to require the tracking of concentration changes of active neurochemicals. In the present study, we demonstrate the feasibility of a method allowing the simultaneous determination of the concentrations of six neurotransmitters: acetylcholine, serotonin, dopamine, gamma-aminobutyric acid (GABA), glutamate and aspartate, in the extracellular brain fluid (EBF). We used hydrophilic interaction chromatography (HILIC) coupled to tandem mass spectrometry (MS/MS) to analyze the EBF from the monkey brain. A push-pull sampling method was used to collect EBF from the prefrontal cortex (PFC) of conscious monkeys at flow rates in the range of low nL/min. The detection limits of acetylcholine, serotonin, dopamine, GABA, glutamate and aspartate were 0.015,0.15,0.3,1.2, 6 and 15 femtomoles, respectively, allowing us to quantitatively determine the concentrations of these six neurotransmitters simultaneously from 500 nL in vivo samples. We conclude that HILIC/MS/MS combined with the push-pull sampling method represents a sensitive technique for simultaneous monitoring of neurotransmitters from EBF samples. Copyright (C) 2007 John Wiley & Sons, Ltd.