The effect of matrix stiffness on biomechanical properties of chondrocytes
The effect of matrix stiffness on biomechanical properties of chondrocytes
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基质刚度对软骨细胞生物力学性能的影响
DOI：
10.1093/abbs/gmw087
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发表时间：
2016-10-01
影响因子：
3.7
通讯作者：
Zhao, Hucheng
中科院分区：
文献类型：
--
作者：
Zhang, Quanyou;Yu, Yang;Zhao, Hucheng
软骨细胞的行为受到多种机械微环境因素的调控。在关节软骨的发育和退行性疾病过程中，软骨细胞的细胞外基质刚度作为一种外部信号发生显著变化，但这种生物物理学信号是否以及如何影响软骨细胞的生物力学特性仍然是一个谜。在本研究中，我们设计了支持生物材料作为模拟天然细胞周围基质，研究基质刚度对软骨细胞形态和F-肌动蛋白分布的影响。此外，利用原子力显微镜技术和理论模型定量研究了软骨细胞在感知和响应不同基质刚度时的主动力学行为。结果表明，较硬的基质有利于增加软骨细胞的铺展面积、不规则细胞形状分布的百分比以及软骨细胞的弹性模量（E-elastic）、瞬时模量（E-0）、松弛模量（E-R）和表观粘度（mu）等力学参数。软骨细胞的基质刚度依赖性生物力学行为的知识具有重要的意义，优化基质材料和推进软骨细胞为基础的功能性组织工程的应用。
The behavior of chondrocytes is regulated by multiple mechanical microenvironmental cues. During development and degenerative disease of articular cartilage, as an external signal, the extracellular matrix stiffness of chondrocytes changes significantly, but whether and how this biophysical cue affects biomechanical properties of chondrocytes remain elusive. In the present study, we designed supporting-biomaterials as mimics of native pericellular matrix to study the effect of matrix stiffness on chondrocyte morphology and F-actin distribution. Furthermore, the active mechanical behavior of chondrocytes during sensing and responding to different matrix stiffness was quantitatively investigated using atom force microscope technique and theoretical model. Our results indicated that stiffer matrix tends to increase the cell spreading area, the percentage of irregular cell shape distribution and mechanical parameters including elastic modulus (E-elastic), instantaneous modulus (E-0), relaxed modulus (E-R) and apparent viscosity (mu) of chondrocytes. Knowledge of matrix stiffness-dependent biomechanical behaviors of chondrocytes has important implications for optimizing matrix material and advancing chondrocyte-based applications for functional tissue engineering.