Extracellular perfusion controller for electrophysiology.
Extracellular perfusion controller for electrophysiology.
复制标题
用于电生理学的细胞外灌注控制器。
DOI：
10.1016/0165-0270(92)90023-7
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发表时间：
1992
影响因子：
3
通讯作者：
Stimers,JR
中科院分区：
文献类型：
--
作者：
Stimers,JR
本文描述了灌注控制器的设计和实用性，以方便处理多种细胞外溶液。该控制器由一个具有手动和计算机控制功能的接口盒以及一组电磁阀组成。这提供了将多种解决方案切换到实验室的远程处理。描述了如何将该系统用于全细胞膜片钳实验来测量各种离子通道电流以及 Na/K 泵电流。讨论了其他可能的用途以及将灌注控制器与现有灌注系统连接的建议。
This paper describes the design and utility of a perfusion controller to facilitate the handling of multiple extracellular solutions. The controller consists of an interface box with both manual and computer controls and a set of solenoid valves. This provides remote handling of the switching of multiple solutions into an experimental chamber. It is described how this system can be used in whole-cell patch-clamp experiments to measure various ion channel currents as well as Na/K pump currents. Other possible uses are discussed along with suggestions for interfacing the perfusion controller with existing perfusion systems.