Capillary and microelectrophoretic separations of ligase detection reaction products produced from low-abundant point mutations in genomic DNA
Capillary and microelectrophoretic separations of ligase detection reaction products produced from low-abundant point mutations in genomic DNA
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DOI：
10.1002/elps.200405886
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发表时间：
2004-06-01
期刊：
影响因子：
2.9
通讯作者：
Barany, F
中科院分区：
文献类型：
--
作者：
Thomas, G;Sinville, R;Barany, F
对毛细管凝胶电泳（CGE）和基于聚合物的微电泳平台进行了研究，以分析某些对结直肠癌具有高诊断价值的基因片段中的低丰度点突变。电泳分离是在由等位基因特异性连接测定（连接酶检测反应，LDR）产生的单链DNA（ssDNA）产物上进行的，该测定用于筛选K - ras癌基因中第12密码子的单碱基突变。突变的存在产生了长度>40个碱基对（bp）的单链DNA片段，而用于连接测定的引物是……
Capillary gel electrophoresis (CGE) and polymer-based microelectrophoretic platforms were investigated to analyze low-abundant point mutations in certain gene fragments with high diagnostic value for colorectal cancers. The electrophoretic separations were carried out on single-stranded DNA (ssDNA) products generated from an allele-specific ligation assay (ligase detection reaction, LDR), which was used to screen for a single base mutation at codon 12 in the K-ras oncogene. The presence of the mutation generated a ssDNA fragment that was >40 base pairs (bp) in length, while the primers used for the ligation assay were