HIV-associated nephropathy: Protocol and rationale for an exploratory genotype-phenotype study in a sub-Saharan African population.
HIV-associated nephropathy: Protocol and rationale for an exploratory genotype-phenotype study in a sub-Saharan African population.
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DOI：
10.1371/journal.pone.0249567
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Aliyu MH
中科院分区：
文献类型：
--
作者：
Yusuf AA;Musa BM;Galadanci NA;Babashani M;Mohammed AZ;Ingles DJ;Fogo AB;Wester CW;Aliyu MH
HIV-positive persons of African descent are disproportionately affected by chronic kidney disease (CKD). Deterioration to end-stage kidney disease (ESKD) also occurs in this population at a higher frequency. There remains a lot to learn about the genetic susceptibility to CKD in HIV positive patients, and the pathophysiology of progression to ESKD. We will conduct an exploratory genotype-phenotype study in HIV-positive persons with CKD in Aminu Kano Teaching Hospital, Nigeria, to determine blood-based differential gene expression biomarkers in different kidney risk groups according to the KDIGO 2012 criteria. We will consecutively screen 150 HIV-positive adults (≥18 years of age) attending the HIV clinic of Aminu Kano Teaching Hospital, Kano, Nigeria, for CKD based on proteinuria and elevation of estimated glomerular filtration rate. Among these, two separate groups of 16 eligible participants each (n = 32) will be selected in the four (4) KDIGO 2012 kidney risk categories. The groups will be matched for age, sex, viral suppression level and antiretroviral (ARV) regimen. In the first group (n = 16), we will determine differential gene expression markers in peripheral blood mononuclear cells using mRNA-sequencing (RNA-Seq). We will validate the differential expression markers in the second group (n = 16) using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Using a systems-based approach, we will construct, visualize and analyze gene-gene interaction networks to determine the potential biological roles of identified differential expression markers based on published literature and publicly available databases. Our exploratory study will provide valuable information on the potential roles of differential expression biomarkers in the pathophysiology of HIV-associated kidney disease by identifying novel biomarkers in different risk categories of CKD in a sub-Saharan African population. The results of this study will provide the basis for population-based genome-wide association studies to guide future personalized medicine approaches. Validated biomarkers can be potential targets for the development of stage-specific therapeutic interventions, an essential paradigm in precision medicine.
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DOI：
10.14740/jocmr3235w
发表时间：
2018-01
期刊：
Journal of clinical medicine research
影响因子：
--
作者：
Husain NE;Ahmed MH;Almobarak AO;Noor SK;Elmadhoun WM;Awadalla H;Woodward CL;Mital D
通讯作者：
Mital D
影响因子：
8.8
作者：
Armenia, Daniele;Zaccarelli, Mauro;Santoro, Maria Mercedes
通讯作者：
Santoro, Maria Mercedes
影响因子：
15.9
作者：
He, JCJ;Husain, M;Klotman, PE
通讯作者：
Klotman, PE
影响因子：
6.4
作者：
Ockenhouse, CF;Bernstein, WB;Vahey, MT
通讯作者：
Vahey, MT
影响因子：
3.3
作者：
Wyatt, Christina M.;Klotman, Paul E.;D'Agati, Vivette D.
通讯作者：
D'Agati, Vivette D.