Taxonomy of Three Oxytrichids (Protozoa, Ciliophora, Hypotrichia) With Establishment of the New Species Rubrioxytricha guangzhouensis spec. nov.
Taxonomy of Three Oxytrichids (Protozoa, Ciliophora, Hypotrichia) With Establishment of the New Species Rubrioxytricha guangzhouensis spec. nov.
复制标题
三种尖毛纲动物（原生动物、纤毛纲、短毛纲）的分类及新种红毛毛虫广州种的建立。
DOI：
10.3389/fmars.2020.623436
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发表时间：
2021-01-27
影响因子：
3.7
通讯作者：
Song, Weibo
中科院分区：
文献类型：
--
作者：
Luo, Xiaotian;Huang, Jie;Song, Weibo
基于活体观察、protargol 制剂和分子分析，对从中国采集的两种尖毛虫纤毛虫，Oxytricha lithofera (Foissner, 2016) 和红红毛虫血浆虫 (Rubrioxytricha haematoplasma) (Blatterer 和 Foissner, 1990; Berger, 1999) 进行了研究。石竹中国种群与模式种群具有高度独特的特征，如岩石体、毗邻的大核结节、直波状的膜和长的背毛。我们新分离的血浆菌的形态和形态发生与模式群体非常一致。获得了两个物种的18S rRNA基因序列，并分析了Lamouroux等人，1824年的Oxytricha Bory de Saint-Vincent属和Berger，1999年的Rubrioxytricha属的分子系统发育。此外，我们发现陈等人描述的广州血胞菌种群。 （2015）可以通过红棕色皮质颗粒（相对于柠檬黄色到绿色）和线粒体样颗粒（相对于不存在），以及微红色细胞质（相对于淡橙色）和海洋或咸水栖息地（相对于淡水）来区分模式群体和我们的分离株。此外，它们的 18S rRNA 基因序列存在 20 bp 的差异，表明它们不是同种的。因此，我们为R. haematoplasma sensu Chen等人建立了一个新种，Rubrioxytricha guangzhouensis。 （2015）。
Two oxytrichid ciliates collected from China, Oxytricha lithofera (Foissner, 2016) and Rubrioxytricha haematoplasma (Blatterer and Foissner, 1990; Berger, 1999), were investigated based on living observations, protargol preparations, and molecular analyses. The Chinese population of O. lithofera shares highly distinctive features with the type population, e.g., lithosomes, abutting macronuclear nodules, straight undulating membranes, and long dorsal bristles. The morphology and morphogenesis of our new isolate of R. haematoplasma corresponds well with the type population. The 18S rRNA gene sequences of both species were obtained, and the molecular phylogeny of the genera Oxytricha Bory de Saint-Vincent in Lamouroux et al., 1824 and Rubrioxytricha (Berger, 1999) was analyzed. In addition, we found that the Guangzhou population of R. haematoplasma described by Chen et al. (2015) can be distinguished from the type population and our isolate by the reddish-brown cortical granules (vs. lemon yellowish to greenish) and mitochondria-like granules present (vs. absent), as well as the slightly red cytoplasm (vs. slightly orange) and the marine or brackish water habitat (vs. freshwater). In addition, a 20-bp divergence in their 18S rRNA gene sequences indicates that they are not conspecific. Thus, we establish a new species, Rubrioxytricha guangzhouensis, for R. haematoplasma sensu Chen et al. (2015).