Enhanced Infrared Neural Stimulation using Localized Surface Plasmon Resonance of Gold Nanorods
Enhanced Infrared Neural Stimulation using Localized Surface Plasmon Resonance of Gold Nanorods
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DOI：
10.1002/smll.201400599
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发表时间：
2014-10-15
期刊：
影响因子：
13.3
通讯作者：
Kim, Sung June
中科院分区：
文献类型：
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作者：
Eom, Kyungsik;Kim, Jinhyung;Kim, Sung June
Kyungsik Eom、Jinhyung Kim、Jong Min Choi、Taekyeong Kang、Jin Woo Chang、Kyung Min Byun、* Sang Beom Jun * 和Sung June Kim* 直接光路，并用于改进耳蜗植入系统。[10还有趣的是，注意到成功的INS方法伴随着神经元附近的温度升高。它暗示神经元质膜的局部热加热触发了动作电位。虽然其机制尚不清楚，但已经假设神经元细胞的去极化与通过神经元的热加热和/或膜电导或电容的改变而激活温度敏感性离子通道（例如TRPV通道）有关。[6，12-14]最近，包括纳米球、纳米棒和纳米壳的各种形状的金纳米颗粒由于其独特的光学性质而经常用于提供光诱导功能，称为局部表面等离子体（LSP）。[15在它们的谐振频率下的照明导致有效的光吸收，其中吸收的能量被转换成热。[17]与由平面金表面支持的传播表面等离子体不同，纳米粒子等离子体诱导量子化的电子振荡局限于纳米级体积，提供了一种操纵光-物质相互作用的方法。因此，等离子体激元触发的局部热可以激活温度敏感的离子通道并引起钙离子的流入，从而以高空间精度刺激神经元。有趣的是，据报道，激光照射与金纳米棒（GNRs）结合在神经元细胞中可以刺激分化，特别是在神经突生长增加方面。[18]这似乎与GNRs中LSP模式的激发引起的瞬态加热有关，这可以促进细胞代谢活性。虽然结果是值得注意的，使用连续波模式和较长的照射时间有麻烦的潜在组织损伤和施加严重的限制上的重复neural excitation.In这项研究中，我们打算证明一种先进的光刺激策略的基础上脉冲INS和等离子GNRs。与以往的方法相比，我们发现，建议的方法是更有利的神经响应度，刺激效率和空间分辨率方面，特别是，可以减少所需的辐射暴露水平，减轻组织损伤的关注。因此，预计我们可以开辟新的可能性，应用于非侵入性调查的各种兴奋组织和治疗神经系统疾病。
Kyungsik Eom, Jinhyung Kim, Jong Min Choi, Taekyeong Kang, Jin Woo Chang, Kyung Min Byun,* Sang Beom Jun,* and Sung June Kim* direct optical path and be utilized to improve a cochlear implant system.[10, 11] It is also interesting to note that successful INS approach is accompanied by temperature elevation in the vicinity of neurons. It implicates the local thermal heating at neuronal plasma membrane for the trigger of action potentials. While the underlying mechanism is not clarified yet, it has been hypothesized that the depolarization of neuron cell is associated with an activation of temperaturesensitive ion channels (eg, TRPV channels) by the thermal heating of neurons and/or the change of the membrane conductance or capacitance.[6, 12–14] Recently, gold nanoparticles of various shapes including nanospheres, nanorods, and nanoshells have been frequently used to offer a light-induced functionality due to their unique optical property, called localized surface plasmons (LSPs).[15, 16] Illumination at their resonant frequency leads to an efficient light absorption, where the absorbed energy is converted to heat.[17] Unlike propagating surface plasmons supported by a planar gold surface, nanoparticle plasmons induce the quantized electron oscillations confined to nanoscale volume, providing a means for manipulating light-matter interaction. Hence, plasmonically triggered local heat can activate the temperature-sensitive ion channels and cause an influx of calcium ions, therefore stimulating the neurons with a high spatial precision. Intriguingly, it was reported that laser exposure combined with gold nanorods (GNRs) in neuronal cells can stimulate differentiation, particularly with regard to an increase in neurite outgrowth.[18] It appeared to be linked to transient heating arising from an excitation of LSP mode in GNRs, which can promote cell metabolic activity. While the results are noteworthy, use of a continuous wave mode and a long irradiation time has trouble with a potential tissue damage and imposes serious constraints on a repetitive neural excitation.In this study, we intend to demonstrate an advanced optical stimulation strategy based on pulsed INS and plasmonic GNRs. Compared with previous approaches, we find that the suggested method is more advantageous in terms of neural responsivity, stimulation efficiency and spatial resolution, and in especial, can reduce the requisite radiant exposure level and alleviate the concern of tissue damage. It is therefore expected that we could open up new possibilities for applications to non-invasive investigations of diverse excitable tissues and treatments of neurological disorders.