A sodium requirement for mitogen-induced proliferation in human peripheral blood lymphocytes.
A sodium requirement for mitogen-induced proliferation in human peripheral blood lymphocytes.
复制标题
人外周血淋巴细胞中丝裂原诱导的增殖需要钠。
DOI：
10.1016/0014-4827(81)90015-x
复制
发表时间：
1981
影响因子：
3.7
通讯作者：
C. Johansson
中科院分区：
文献类型：
--
作者：
C. Deutsch;M. Price;C. Johansson
丝裂原刺激人外周血淋巴细胞DNA合成依赖于细胞外Na+。DNA合成也受到类似的抑制：1.1。悬浮在低渗培养基中的细胞含有减少的细胞外Na。将细胞悬浮在含有降低的Na和等摩尔的胆碱替代的培养基中。将细胞悬浮在含有低Na和等渗甘露醇替代的培养基中，即使淋巴细胞仅暴露于低Na 3 h，然后恢复到正常Na水平，在第3天也观察到PHA诱导的DNA合成减少。我们对蛋白质合成的研究表明，细胞外Na降低对DNA合成和细胞分裂的影响并不是因为初始抑制了整体蛋白质合成。这些数据表明，减少外部Na对淋巴细胞有丝分裂发生中的某些特定早期事件（~ 3 h）具有显著影响。
Mitogen-stimulated DNA synthesis in human peripheral blood lymphocytes is dependent on extracellular Na. DNA synthesis was similarly inhibited in:1.1. Cells that were suspended in hypotonic media containing decreased extracellular Na.2.2. Cells that were suspended in media containing decreased Na and equimolar replacement with choline.3.3. Cells that were suspended in media containing decreased Na and equiosmolar replacement with mannitol.A decreased PHA-induced DNA synthesis was observed at day 3 even when lymphocytes were exposed to low Na for only the first 3 h and then returned to normal levels of Na. Our studies of protein synthesis indicate that the effect of lowered extracellular Na on DNA synthesis and cell division is not due to an initial inhibition of overall protein synthesis. These data suggest that reduced external Na has a significant effect on some specific early event(s) (⩽3 h) in lymphocyte mitogenesis.