Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy
Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy
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DOI：
10.1002/jemt.20178
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发表时间：
2005-05-01
影响因子：
2.5
通讯作者：
Girkin, JM
中科院分区：
文献类型：
--
作者：
Wright, AJ;Burns, D;Girkin, JM
我们报道了在共焦和多光子显微镜中引入有源光学元件以减小样品引起的像差。使用一个灵活的膜镜作为有源元件，进入显微镜物镜的后面的光束被改变，以产生最小的点扩散函数，一旦它被带到一个焦点内的样品。自适应光学的传统方法通常用于天文学，是利用波前传感器来确定所需的反射镜形状。我们已经开发了一种技术，使用优化算法来改善返回的信号，而不使用波前传感器。我们已经研究了一些可能的优化方法，包括爬山法，遗传算法和更多的随机搜索方法。该系统已被证明是一个显着的增强共焦显微镜的轴向分辨率时，在样品内的深度成像。我们讨论的权衡采用的各种方法，比较速度与分辨率增强。(c)2005 Wilev-Liss，Inc.
We report on the introduction of active optical elements into confocal and multiphoton microscopes in order to reduce the sample-induced aberration. Using a flexible membrane mirror as the active element, the beam entering the rear of the microscope objective is altered to produce the smallest point spread function once it is brought to a focus inside the sample. The conventional approach to adaptive optics, commonly used in astronomy, is to utilise a wavefront sensor to determine the required mirror shape. We have developed a technique that uses optimisation algorithms to improve the returned signal without the use of a wavefront sensor. We have investigated a number of possible optimisation methods, covering hill climbing, genetic algorithms, and more random search methods. The system has demonstrated a significant enhancement in the axial resolution of a confocal microscope when imaging at depth within a sample. We discuss the trade-offs of the various approaches adopted, comparing speed with resolution enhancement. (c) 2005 Wilev-Liss, Inc.