Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP.
Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP.
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DOI：
10.1007/978-1-0716-2132-5_21
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Shanjin Huang
中科院分区：
文献类型：
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作者：
Min Diao;Shanjin Huang
Plasmodesmata (PD) play an important role in plant growth and development and defense. The permeability of PD is strictly regulated. Here, we describe an assay for measuring the permeability of PD in Arabidopsis thaliana leaves, which relies on tracing intercellular movement of green fluorescent protein (GFP) upon transient expression of the protein-encoding plasmid delivered by particle bombardment. The method allows to assess GFP movement at single-cell resolution.
