Multiple mechanisms involved in the inhibition of proinflammatory cytokine production from human monocytes by N-(p-coumaroyl)serotonin and its derivatives
Multiple mechanisms involved in the inhibition of proinflammatory cytokine production from human monocytes by N-(p-coumaroyl)serotonin and its derivatives
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DOI：
10.1016/s1567-5769(02)00207-2
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发表时间：
2003-02-01
影响因子：
5.6
通讯作者：
Onozaki, K
中科院分区：
文献类型：
--
作者：
Takii, T;Kawashima, S;Onozaki, K
本文报道了从红花油饼（Carthamus tinctorius L.）抑制内毒素（LPS）刺激的人单核细胞产生促炎细胞因子。在这项研究中，CS及其三种衍生物，N-（trans-cinnamoyl）serotonin（Cin.S），N-（trans-cinnamoyl）tryptamine（Cin.T）和N-（p-coumaroyl）tryptamine（CT）对促炎细胞因子的产生的影响进行了比较。Cin.S具有与CS相当的自由基清除活性，而CT和Cin.T表现出较低的活性，这表明5-羟色胺中的羟基是必不可少的抗氧化活性。CS和CT强烈抑制LPS刺激的人单核细胞产生促炎细胞因子（IL-1 α、IL-1 β、IL-6、IL-8和TNF-α）。然而，Cin.S仅抑制IL-1 α和IL-1 β的产生，而Cin.T不抑制这些细胞因子的产生。CS和CT明显抑制单核细胞蛋白质合成，Cin.S抑制作用中等，Cin.T抑制作用较弱。这些结果表明，CS及其衍生物通过多种机制抑制促炎细胞因子的产生。(C)2002 Elsevier Science B. V.保留所有权利。
We have reported that N-(p-coumaroyl)serotonin(CS) isolated from safflower oil cake (Carthamus tinctorius L.) inhibits the production of proinflammatory cytokines by endotoxin (LPS)-stimulated human monocytes. In this study, the effects of CS and its three derivatives, N-(trans-cinnamoyl)serotonin (Cin.S), N-(trans-cinnamoyl)tryptamine (Cin.T), and N-(p-coumaroyl)tryptamine (CT) on the production of proinflammatory cytokines were compared. Cin.S possessed radical scavenging activity at a comparable level to CS, while CT and Cin.T exhibited lower activity, suggesting that hydroxyl group in serotonin is essential for the antioxidative activity. CS and CT strongly inhibited the production of proinflammatory cytokines (IL-1alpha, IL-1beta, IL-6, IL-8, and TNF-alpha) from LPS-stimulated human monocytes. However, Cin.S inhibited the production of only IL-1alpha and IL-1beta, and Cin.T inhibited none of these cytokines production. CS and CT markedly inhibited the protein synthesis in monocytes, the inhibitory effect of Cin.S was moderate, and that of Cin.T was quite weak. These results indicate that CS and its derivatives inhibit the production of proinflammatory cytokines through multiple mechanisms. (C) 2002 Elsevier Science B.V. All rights reserved.