Unknown peptide sequencing using matrix-assisted laser desorption/ionization and in-source decay.
Unknown peptide sequencing using matrix-assisted laser desorption/ionization and in-source decay.
复制标题
使用基质辅助激光解吸/电离和源内衰变进行未知肽测序。
DOI：
10.1021/ac971158d
复制
发表时间：
1998
影响因子：
7.4
通讯作者：
Bailey,J
中科院分区：
文献类型：
--
作者：
Reiber,DC;Brown,RS;Weinberger,S;Kenny,J;Bailey,J
研究的结果，以确定在源衰变碎片的基质辅助激光解吸离子的效用，以获得有用的序列信息未知的肽。通过高效液相色谱法纯化六种肽，并作为单盲未知物提交。源内衰变碎片离子数据收集在配备延迟提取的线性飞行时间质谱仪上。根据已知的碎片化途径手动解释这些碎片离子数据，以确定拟定序列。其中三个未知数的建议序列基本上是正确的，有一些小错误。由于对碎片化数据的误解，第四个未知序列与其拟定序列相关存在重大错误。发现两个未知样品在分析前发生了显著的样品降解，这影响了这些样品的结果。还提供了使用蛋白质数据库搜索部分肽序列以帮助序列测定的实例。
The results of a study to determine the utility of in-source decay fragmentation of matrix-assisted laser-desorbed ions for obtaining useful sequence information on unknown peptides are presented. Six peptides were purified by high-performance liquid chromatography and submitted as single blind unknowns. The in-source decay fragment ion data were collected on a linear time-of-flight mass spectrometer equipped with delayed extraction. These fragment ion data were manually interpreted on the basis of known fragmentation pathways to determine a proposed sequence. The proposed sequences for three of the unknowns were essentially correct, with a few minor errors. A fourth unknown had significant errors associated with its proposed sequence due to misinterpretation of the fragmentation data. Two unknowns were found to have undergone significant sample degradation prior to analysis, which compromised the results for these samples. An example of the use of protein database searching of a partial peptide sequence to aid in a sequence determination is also presented.