Cholesterol promotes hemifusion and pore widening in membrane fusion induced by influenza hemagglutinin.
Cholesterol promotes hemifusion and pore widening in membrane fusion induced by influenza hemagglutinin.
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DOI：
10.1085/jgp.200709932
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发表时间：
2008-05
影响因子：
3.8
通讯作者：
Zimmerberg, Joshua
中科院分区：
文献类型：
--
作者：
Biswas, Subrata;Yin, Shu-Rong;Blank, Paul S.;Zimmerberg, Joshua
使用昆虫细胞测试影响膜融合的胆固醇特异性相互作用;具有天然低胆固醇水平（<4mol%）的细胞。将Sf 9细胞（HAS细胞）工程化以表达流感病毒X-31株的血凝素（HA）。用胆固醇富集HAS细胞减少了HAS细胞和人红细胞（RBC）之间的触发和脂质染料转移之间的延迟，表明胆固醇促进融合孔开放之前的膜脂质混合。增加的胆固醇也增加了在宽范围的HA表达水平上HAS细胞和RBC之间的含水量转移，这表明胆固醇也有利于融合孔扩张。在富含胆固醇的细胞中使用跨细胞染料扩散和融合孔电导率测量来测试这种解释。本研究的结果支持宿主细胞胆固醇在膜融合中的两个阶段起作用的假设：（1）早期，在融合孔开放之前，和（2）晚期，在融合孔扩张期间。
Cholesterol-specific interactions that affect membrane fusion were tested for using insect cells; cells that have naturally low cholesterol levels (<4 mol %). Sf9 cells were engineered (HAS cells) to express the hemagglutinin (HA) of the influenza virus X-31 strain. Enrichment of HAS cells with cholesterol reduced the delay between triggering and lipid dye transfer between HAS cells and human red blood cells (RBC), indicating that cholesterol facilitates membrane lipid mixing prior to fusion pore opening. Increased cholesterol also increased aqueous content transfer between HAS cells and RBC over a broad range of HA expression levels, suggesting that cholesterol also favors fusion pore expansion. This interpretation was tested using both trans-cell dye diffusion and fusion pore conductivity measurements in cholesterol-enriched cells. The results of this study support the hypothesis that host cell cholesterol acts at two stages in membrane fusion: (1) early, prior to fusion pore opening, and (2) late, during fusion pore expansion.
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DOI：
10.1083/jcb.200412058
发表时间：
2005-06-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hess ST;Kumar M;Verma A;Farrington J;Kenworthy A;Zimmerberg J
通讯作者：
Zimmerberg J
影响因子：
3.4
作者：
Feigenson, GW;Buboltz, JT
通讯作者：
Buboltz, JT
影响因子：
4.5
作者：
Frolov, VA;Cho, MS;Zimmerberg, J
通讯作者：
Zimmerberg, J
影响因子：
3.4
作者：
Frolov, VA;Dunina-Barkovskaya, AY;Zimmerberg, J
通讯作者：
Zimmerberg, J
影响因子：
4
作者：
Churchward, MA;Rogasevskaia, T;Coorssen, JR
通讯作者：
Coorssen, JR