RNA-Seq-based transcriptome and the reproduction-related genes for the aphid Schlechtendalia chinensis (Hemiptera, Aphididae)
RNA-Seq-based transcriptome and the reproduction-related genes for the aphid Schlechtendalia chinensis (Hemiptera, Aphididae)
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基于RNA-Seq的蚜虫Schlechtendalia chinensis（半翅目，蚜科）转录组和繁殖相关基因
DOI：
10.4238/gmr16019448
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发表时间：
2017-03-24
影响因子：
0.4
通讯作者：
Yang, P.
中科院分区：
文献类型：
--
作者：
Liu, P.;Yang, Z. X.;Yang, P.
中国瘿形成于漆树植物（盐肤木），由于其富含单宁，用于医药和化学目的。虫瘿由蚜虫形成，其中最突出的是中国长角蚜（Schlechtendalia chinensis），它在R. chinensis。S. chinensis生活史复杂，包括R.中国和某些苔藓作为宿主，以及存在有性和无性生殖（周期性孤雌生殖）。以往的研究表明，有性生殖和无性生殖的交替发生依赖于许多环境因素，如温度，光周期和寄主植物。然而，在分子水平上对有性和无性生殖模式知之甚少。我们的目的是确定基因，响应温度的变化，可能与繁殖过程有关。我们比较了两个S. chinensis，在不同温度下饲养。利用基因本体论分析，共鉴定出51个与昆虫生殖决定相关的进化保守基因。在这些基因中，S. chinensis有42个基因。当我们分析具有无性或有性繁殖后代的蚜虫时，这42个基因中有8个被鉴定出来，并且在两种温度条件下表达不同。这是首次报道与生殖决定相关的基因。chinensis，具有复杂的生命周期。不同温度条件下基因表达的差异将有助于理解蚜虫生殖决定机制。
Chinese galls form on sumac plants (Rhus chinensis) and are used for medicinal and chemical purposes, due to their richness in tannins. The galls are formed by aphids, the most prominent of which is Schlechtendalia chinensis, which forms horn-shaped galls on the winged rachis of R. chinensis. S. chinensis has a complex life cycle, including both R. chinensis and certain mosses as hosts, as well as the existence of both sexual and asexual reproduction (cyclical parthenogenesis). Previous studies have shown that the alternate occurrence of sexual and asexual reproduction relies on many environmental factors, such as temperature, photoperiod, and host-plant. However, the sexual and asexual modes of reproduction are poorly understood on the molecular level. We aimed to identify genes that respond to changes in temperature that may be related to the reproduction process. We compared the transcriptome of two samples of S. chinensis, which had been reared at different temperatures. Using gene ontology analysis, a total of 51 evolutionary conserved genes related to reproduction determination in insects were identified. Of these genes, S. chinensis harbors 42 genes. When we analyzed aphids that had asexually or sexually produced offspring, eight out of these 42 genes were identified and expressed differently in two temperature conditions. This is the first report on genes associated with reproduction determination in S. chinensis, which has a complex life cycle. Genes, expressed differently in response to different temperature conditions will be helpful to understand the mechanism of aphid reproductive determination.