Monosomy 3 in uveal melanoma: Correlation with clinical and histologic predictors of survival
Monosomy 3 in uveal melanoma: Correlation with clinical and histologic predictors of survival
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DOI：
10.1167/iovs.02-0159
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发表时间：
2003-03-01
影响因子：
4.4
通讯作者：
Field, JK
中科院分区：
文献类型：
--
作者：
Scholes, AGM;Damato, BE;Field, JK
目的.葡萄膜黑色素瘤3号单体与临床和组织学预后变量及转移性疾病引起的死亡相关。应用PCR微卫星分析技术研究了105例肿瘤3号染色体上的杂合性缺失（洛），并分析了LOH与肿瘤基底直径（LBD）、睫状体（CB）受累、肿瘤细胞类型、PAS阳性环以及转移性疾病相关死亡的关系。一个模型与单体3，这些被创建与正向逐步逻辑回归，并用于获得预后指数。54例（51%）肿瘤发生3号单体，6例（6%）肿瘤发生3号染色体区域性洛缺失。单体3与上皮样细胞（χ 2检验，P < 0.001）、PAS阳性环（χ 2检验，P = 0.001）、LBD（Mann-Whitney检验，P = 0.002）、CB受累（χ 2检验，P = 0.008）和转移相关死亡（对数秩分析，P = 0.0003）相关。回归系数表明上皮组织学。LBD每增加一毫米，其影响力就增加15倍。得出预后评分：每个LBD类别（17.4 mm）为1分，上皮组织学为3分。3号单体的发生率随着评分的增加而增加，从18个评分小于4的肿瘤的0%增加到21个评分为7的肿瘤的95%。单体3与生存率相关，但仅在大的上皮样肿瘤患者中可以预测。3号单体的缺失仅在小的梭形细胞肿瘤患者中是可预测的。在大多数患者中，根据肿瘤大小和组织学预测单体3是不可靠的。
PURPOSE. To correlate monosomy 3 in uveal melanoma with clinical and histologic prognostic variables and death caused by metastatic disease.METHODS. Loss of heterozygosity (LOH) on chromosome 3 was investigated by PCR-based microsatellite analysis in 105 tumors and related to large basal tumor diameter (LBD), ciliary body (CB) involvement, tumor cell type, periodic acid-Schiff (PAS)positive loops, and death related to metastatic disease. A model relating monosomy 3 to these was created with forward-stepwise logistic regression and used to derive a prognostic index.RESULTS. Monosomy 3 occurred in 54 (51%) tumors and regional chromosome 3 LOH in another six (6%) tumors. Monosomy 3 was associated with epithelioid cells (chi(2) test, P < 0.001), PAS-positive loops (chi(2), P = 0.001), LBD (Mann-Whitney test, P = 0.002), CB involvement (chi(2) test, P = 0.008), and metastasis-related death (log rank analysis, P = 0.0003). The regression coefficients indicated that epithehoid histology. was 15 times as influential with each millimeter of increase in LBD. A prognostic score was derived: one point for each LBD category (17.4 mm) and three points for epithehoid histology. The prevalence of monosomy 3 increased with score, from 0% in 18 tumors scoring less than 4 to 95% in 21 tumors scoring 7.CONCLUSIONS. Monosomy 3 correlates with survival but can be predicted only in patients with large epithelioid tumors. The absence of monosomy 3 is predictable only in patients who have small, spindle-cell tumors. In most patients, prediction of monosomy 3 according to tumor size and histology is unreliable.