Using Biological Photophysics to Map the Excited-State Topology of Molecular Photosensitizers for Photodynamic Therapy.
Using Biological Photophysics to Map the Excited-State Topology of Molecular Photosensitizers for Photodynamic Therapy.
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利用生物光物理学绘制光动力疗法分子光敏剂的激发态拓扑。
DOI：
10.1002/anie.202301452
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Dietzek-Ivanšić,Benjamin
中科院分区：
文献类型：
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作者：
Chettri,Avinash;Yang,Tingxiang;Cole,HoustonD;Shi,Ge;Cameron,ColinG;McFarland,SherriA;Dietzek-Ivanšić,Benjamin
本研究以人体临床试验中基于RuII的光动力疗法(PDT)试剂TLD1433为基准，建立了在人类癌细胞提供的局部环境中研究光敏剂(PSS)在细胞中的激发态动力学的方案。人们对活细胞中PS的激发态性质知之甚少，对于TLD1433来说，它是未知的。这项贡献针对光疗中的一个普遍问题，即如何在相关的生物环境中询问PSS的光触发、功能决定过程，并建立了研究TLD1433被MCF7细胞摄取时的超快光诱导过程的方法学进展。我们从分子物理学的角度概括了这些方法的发展和结果，并将它们应用于TLD1433的S类似物TLD1633，使本研究成为研究复杂生物环境中光毒性化合物激发态动力学的基准。
This study employs TLD1433, a RuII‐based photodynamic therapy (PDT) agent in human clinical trials, as a benchmark to establish protocols for studying the excited‐state dynamics of photosensitizers (PSs)in cellulo, in the local environment provided by human cancer cells. Very little is known about the excited‐state properties of any PS in live cells, and for TLD1433, it isterra incognita. This contribution targets a general problem in phototherapy, which is how to interrogate the light‐triggered, function‐determining processes of the PSs in the relevant biological environment, and establishes methodological advances to study the ultrafast photoinduced processes for TLD1433 when taken up by MCF7 cells. We generalize the methodological developments and results in terms of molecular physics by applying them to TLD1433’s analogue TLD1633, making this study a benchmark to investigate the excited‐state dynamics of phototoxic compounds in the complex biological environment.