Lycium barbarum polysaccharides reduce intestinal ischemia/reperfusion injuries in rats
Lycium barbarum polysaccharides reduce intestinal ischemia/reperfusion injuries in rats
复制标题
枸杞多糖减轻大鼠肠道缺血/再灌注损伤
DOI：
10.1016/j.cbi.2013.05.010
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发表时间：
2013-08-25
影响因子：
5.1
通讯作者：
Hu, Dahai
中科院分区：
文献类型：
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作者：
Yang, Xuekang;Bai, Hua;Hu, Dahai
炎症和氧化应激在肠缺血再灌注损伤中起重要作用。枸杞多糖（LBP）在不同的模型中显示出有效的抗氧化和免疫调节功能。本研究的目的是评估LBP在肠道IRI中的作用和潜在机制。使用几种自由基产生和脂质过氧化模型来评估LBP的体外抗氧化活性。采用普通的肠缺血再灌注（IRI）模型，通过夹闭和松开上级肠系膜动脉造成大鼠肠损伤。检测丙二醛（MDA）、肿瘤坏死因子（TNF）-α、活化核因子（NF）-κ B、细胞内粘附分子（ICAM）-1、E-选择素和相关抗氧化酶水平、多形核中性粒细胞（PMN）蓄积、肠通透性和肠组织学的变化。我们发现LBPs在体外表现出明显的抗自由基和抗脂质过氧化作用。LBPs增加了肠道IRI动物模型中抗氧化酶的水平，减少了肠道氧化损伤。此外，LBP抑制PMN积聚和ICAM-1表达，并改善TNF-α水平、NF-κ B活化、肠通透性和组织学的变化。我们的研究结果表明，LBP治疗可能通过抑制IR诱导的氧化应激和炎症来预防IR诱导的肠道损伤。(C)2013爱思唯尔爱尔兰有限公司版权所有。
Inflammation and oxidative stress exert important roles in intestinal ischemia-reperfusion injury (IRI). Lycium barbarum polysaccharides (LBPs) have shown effective antioxidative and immunomodulatory functions in different models. The purpose of the present study was to assess the effects and potential mechanisms of LBPs in intestinal IRI. Several free radical-generating and lipid peroxidation models were used to assess the antioxidant activities of LBPs in vitro. A common IRI model was used to induce intestinal injury by clamping and unclamping the superior mesenteric artery in rats. Changes in the malondialdehyde (MDA), tumor necrosis factor (TNF)-alpha, activated nuclear factor (NF)-kappa B, intracellular adhesion molecule (ICAM)-1, E-selectin, and related antioxidant enzyme levels, polymorphonuclear neutrophil (PMN) accumulation, intestinal permeability, and intestinal histology were examined. We found that LBPs exhibited marked inhibitory action against free radicals and lipid peroxidation in vitro. LBPs increased the levels of antioxidant enzymes and reduced intestinal oxidative injury in animal models of intestinal IRI. In addition, LBPs inhibited PMN accumulation and ICAM-1 expression and ameliorated changes in the TNF-alpha level, NF-kappa B activation, intestinal permeability, and histology. Our results indicate that LBPs treatment may protect against IRI-induced intestinal damage, possibly by inhibiting IRI-induced oxidative stress and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved.