Fine mapping of a recessive genic male sterility gene (Bnms3) in rapeseed (Brassica napus) with AFLP- and Arabidopsis-derived PCR markers
Fine mapping of a recessive genic male sterility gene (Bnms3) in rapeseed (Brassica napus) with AFLP- and Arabidopsis-derived PCR markers
复制标题
使用 AFLP 和拟南芥来源的 PCR 标记对油菜（甘蓝型油菜）中的隐性雄性不育基因 (Bnms3) 进行精细定位
DOI：
10.1007/s00122-008-0747-x
复制
发表时间：
2008-06-01
影响因子：
5.4
通讯作者：
Yang, Guangsheng
中科院分区：
文献类型：
--
作者：
He, Junping;Ke, Liping;Yang, Guangsheng
9012 AB，一种由甘蓝型油菜自发突变产生的隐性核雄性不育（RGMS）系（Chen等，Acta Agron Sin 24：431-438，1998），在中国杂种栽培品种开发中发挥着越来越重要的作用。9012 AB的雄性不育性受两对隐性基因（Bnms 3和Bnms 4）和一对隐性上位抑制基因（esp）互作控制。先前的研究已经鉴定了七个AFLP标记，其中六个在小群体中与Bnms 3基因共分离（Ke等人，Plant Breed 124：367-370，2005）。通过对这些AFLP标记及其侧翼序列的克隆，成功地将6个共分离标记中的5个转化为序列特征扩增区（SCAR）标记。为了精细定位Bnms 3基因，在4，136个个体的NIL群体中分析这些SCAR标记。Bnms 3基因定位于0.56 cM的区域，其中0.15 cM来自标记SEP 8，0.41来自标记SEP 4。用这些SCAR标记序列进行的BLAST分析鉴定了拟南芥5号染色体中的共线基因组区域，其中两个特异性PCR标记进一步将Bnms 3基因座从0.56 cM的区间缩小到0.14 cM。这些结果为Bnms 3基因的图位克隆提供了新的信息，并将有助于RGMS优良系和保持系的分子标记辅助选择。
9012AB, a recessive genic male sterility (RGMS) line developed from spontaneous mutation in Brassica napus (Chen et al. in Acta Agron Sin 24:431-438, 1998), has been playing an increasing role in hybrid cultivar development in China. The male sterility of 9012AB is controlled by two recessive genes (designated Bnms3 and Bnms4) interacting with one recessive epistatic suppressor gene (esp). Previous study has identified seven AFLP markers, six of which were co-segregated with the Bnms3 gene in a small population (Ke et al. in Plant Breed 124:367-370, 2005). By cloning these AFLP markers and their flanking sequences, five of the six co-segregated markers were successfully converted into sequence characterized amplified region (SCAR) markers. For fine mapping of the Bnms3 gene, these SCAR markers were analyzed in a NIL population of 4,136 individuals. The Bnms3 gene was then genetically mapped to a region of 0.56 cM, with 0.15 cM from marker SEP8 and 0.41 from marker SEP4, respectively. BLAST analysis with these SCAR marker sequences identified a collinear genomic region in Arabidopsis chromosome 5, from which two specific PCR markers further narrowed the Bnms3 locus from an interval of 0.56 to 0.14 cM. These results provide additional information for map-based cloning of the Bnms3 gene and will be helpful for marker-assisted selection (MAS) of elite RGMS lines and maintainers.