Lytic Susceptibility, Structure, and Mechanical Properties of Fibrin in Systemic Lupus Erythematosus
Lytic Susceptibility, Structure, and Mechanical Properties of Fibrin in Systemic Lupus Erythematosus
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DOI：
10.3389/fimmu.2019.01626
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发表时间：
2019-07-16
影响因子：
7.3
通讯作者：
Weisel, John W.
中科院分区：
文献类型：
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作者：
Litvinov, Rustem;Nabiullina, Rosa M.;Weisel, John W.
在系统性红斑狼疮（SLE）的并发症中，血栓性事件相对常见，并显著增加其发病率和死亡率。各种疾病中血栓形成的风险增加已被证明与由其结构决定的纤维蛋白凝块的溶解稳定性和机械刚度相关。在这里，我们研究了SLE患者的纤维蛋白凝块性质的变化与疾病严重程度的关系。从28例SLE患者的血浆凝块的特点是动力学的形成和纤溶溶解（使用动态比浊法），网络和纤维超微结构（扫描电子显微镜），粘弹性（剪切流变仪），交联的速度和程度（蛋白质印迹法）与疾病的活动，血液成分，并相比，凝血池正常人血浆。外源性t-PA对SLE患者血浆凝块的溶解速度明显快于正常人血浆凝块，活动期（SLEDAI>4）和非活动期（SLEDAI> 4）之间无显著性差异
Among complications of systemic lupus erythematosus (SLE), thrombotic events are relatively common and contribute significantly to the morbidity and mortality rates. An increased risk of thrombosis in various diseases has been shown to be associated with the lytic stability and mechanical stiffness of the fibrin clot determined by its structure. Here we studied alterations of the fibrin clot properties in relation to disease severity in SLE patients. Plasma clots from 28 SLE patients were characterized by the kinetics of formation and fibrinolytic dissolution (using dynamic turbidimetry), the network and fiber ultrastructure (scanning electron microscopy), viscoelasticity (shear rheometry), and the rate and degree of crosslinking (Western blotting) correlated with the disease activity, blood composition, and compared to clotting of pooled normal human plasma. Clots made from plasma of SLE patients were lysed faster with exogenous t-PA than control clots from normal plasma without a significant difference between those from active (SLEDAI>4) and inactive (SLEDAI