Inorganic polyphosphate: a possible stimulant of bone formation
Inorganic polyphosphate: a possible stimulant of bone formation
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DOI：
10.1177/154405910708600917
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发表时间：
2007-09-01
影响因子：
7.6
通讯作者：
Furusawa, K.
中科院分区：
文献类型：
--
作者：
Hacchou, Y.;Uematsu, T.;Furusawa, K.
无机聚磷酸盐[Poly(P)]通常分布在成骨细胞中。我们进行了这项研究，以验证Poly(P)刺激成骨细胞和促进骨形成的假设。用Poly(P)培养成骨样细胞系MC 3T3-E1，用逆转录聚合酶链式反应检测基因表达和潜在矿化。测定碱性磷酸酶活性、von Kossa染色和吸收陷窝形成分析。在大鼠牙槽骨再生模型中评估了Poly(P)在骨形成中的潜在作用。Poly(P)诱导MC 3T3-E1细胞表达骨桥蛋白、骨钙素、1α胶原和护骨素，并增加碱性磷酸酶的活性。在Poly的存在下，小鼠成骨细胞和骨髓巨噬细胞共培养时牙本质片凹陷的形成减少(P)。Poly(P)处理组大鼠牙槽骨再生有明显的促进作用。这些发现表明Poly(P)在成骨细胞分化、激活和骨矿化中起作用。因此，局部注射聚(P)可能对牙周病有治疗作用。
Inorganic polyphosphates [ Poly( P)] are often distributed in osteoblasts. We undertook the present study to verify the hypothesis that Poly( P) stimulates osteoblasts and facilitates bone formation. The osteoblast-like cell line MC 3T3-E1 was cultured with Poly( P), and gene expression and potential mineralization were evaluated by reverse-transcription polymerase chain-reaction. Alkaline phosphatase activity, von Kossa staining, and resorption pit formation analyses were also determined. The potential role of Poly( P) in bone formation was assessed in a rat alveolar bone regeneration model. Poly( P) induced osteopontin, osteocalcin, collagen 1 alpha, and osteoprotegerin expression and increased alkaline phosphatase activity in MC 3T3- E1 cells. Dentin slice pit formation decreased with mouse osteoblast and bone marrow macrophage co-cultivation in the presence of Poly( P). Promotion of alveolar bone regeneration was observed locally in Poly( P)treated rats. These findings suggest that Poly( P) plays a role in osteoblastic differentiation, activation, and bone mineralization. Thus, local poly( P) delivery may have a therapeutic benefit in periodontal disease.