Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers
Extraction and reconstitution of membrane proteins into lipid nanodiscs encased by zwitterionic styrene-maleic amide copolymers
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DOI：
10.1038/s41598-020-66852-7
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发表时间：
2020-06-18
影响因子：
4.6
通讯作者：
Liang, Hongjun
中科院分区：
文献类型：
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作者：
Fiori, Mariana C.;Zheng, Wan;Liang, Hongjun
膜蛋白可以在聚合物包裹的纳米圆盘中重建，以便在接近生理条件下和没有洗涤剂的情况下进行研究，但用于此目的的传统苯乙烯-马来酸共聚物存在严重的缓冲不相容性。我们最近引入了两性离子苯乙烯-马来酰胺共聚物（zsma）来克服这一限制。在这里，我们比较了一系列具有不同苯乙烯：马来酰胺摩尔比、链大小和分子量分布的zsma对膜蛋白的提取和重构成脂质纳米盘的影响。这些共聚物在纳米圆盘中溶解、稳定和支持膜蛋白，其效率取决于共聚物和膜蛋白的结构。
Membrane proteins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological conditions and in the absence of detergents, but traditional styrene-maleic acid copolymers used for this purpose suffer severely from buffer incompatibilities. We have recently introduced zwitterionic styrene-maleic amide copolymers (zSMAs) to overcome this limitation. Here, we compared the extraction and reconstitution of membrane proteins into lipid nanodiscs by a series of zSMAs with different styrene:maleic amide molar ratios, chain sizes, and molecular weight distributions. These copolymers solubilize, stabilize, and support membrane proteins in nanodiscs with different efficiencies depending on both the structure of the copolymers and the membrane proteins.