Frequent False-Positive Reactions in Pronase-Treated T-Cell Flow Cytometric Cross-match Tests
Frequent False-Positive Reactions in Pronase-Treated T-Cell Flow Cytometric Cross-match Tests
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DOI：
10.1016/j.transproceed.2011.12.048
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发表时间：
2012-01-01
影响因子：
0.9
通讯作者：
Park, M. H.
中科院分区：
文献类型：
--
作者：
Park, H.;Lim, Y. M.;Park, M. H.
目标。移植前交叉配型（XM）在器官移植中是必不可少的。流式细胞术（FCXM）是最灵敏的基于细胞的XM技术。Pronase处理可提高b细胞FCXM的敏感性和特异性。因此，pronase处理（PT）的T细胞在单管T细胞/ b细胞技术中进行测试。观察PT与未治疗（PN） T-FCXM结果的差异，探讨其发生率、临床意义及可能的原因。我们使用PT和PN T-FCXM分别使用3色和2色免疫荧光染色对167名肾移植候选人或移植后随访患者的226份血清样本进行了检测。我们回顾了小组反应性抗体（PRA）和供体特异性抗体（DSA）状况以及HLA数据和临床结果。T-FCXM阳性率在PT和PN试验中显著高于(24.3%比11.1%
Objective. Pretransplantation cross-match (XM) is essential in organ transplantation. The flow cytometric XM (FCXM) is the most sensitive cell-based XM technique. Pronase treatment is used to improve the sensitivity and specificity of the B-cell FCXM. Thus, pronase-treated (PT) T cells are tested in a single tube T-cell/B-cell technique. Observing discrepancies between PT and pronase-nontreated (PN) T-FCXM results, we investigated their incidence, clinical significance, and possible causes.Methods. We tested 226 serum samples from 167 kidney transplantation candidates or posttransplantation follow-up patients using PT and PN T-FCXM in parallel using 3-color and 2-color immunofluorescence staining, respectively. We reviewed panel-reactive antibody (PRA) and donor-specific antibody (DSA) status as well as HLA data and clinical outcomes.Results. The T-FCXM positive rate was significantly higher among PT versus PN tests (24.3% vs 11.1%; P