Previously unrecognized stages of species-specific colonization in the mutualism between Xenorhabdus bacteria and Steinernema nematodes.
Previously unrecognized stages of species-specific colonization in the mutualism between Xenorhabdus bacteria and Steinernema nematodes.
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DOI：
10.1111/cmi.12134
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发表时间：
2013-09
影响因子：
3.4
通讯作者：
Goodrich-Blair H
中科院分区：
文献类型：
--
作者：
Chaston JM;Murfin KE;Heath-Heckman EA;Goodrich-Blair H
水平传播的微生物共生的特异性通常由初始接合期间宿主和微生物之间的分子通信来定义，其可以在离散的阶段中发生。在斯氏线虫线虫和Xabrihabdus细菌之间的共生关系，以前的调查集中在细菌定植的肠腔（插座）的线虫感染的少年（IJ），因为这是唯一已知的持久，亲密，和物种之间的特定接触。在这里，我们表明，细菌殖民的前肠细胞的其他线虫发育阶段，在一个物种特异性的方式。此外，我们描述了三个过程，只发生在青少年注定要成为IJs。首先，一些细菌细胞定殖线虫咽肠瓣（PIV）前面的肠上皮。第二，线虫肠道收缩，而细菌最初留在PIV中。第三，前肠收缩松弛，定植细菌占据容器。在每个阶段，殖民化都需要X。SR1基因具有种特异性：X.天然缺乏SR1的szentirmaii不会定殖，除非SR1异位表达。这些研究结果揭示了新的方面，Xabridus细菌的相互作用和传播的斯氏线虫宿主，并证明细菌SR1基因有助于在定居线虫上皮表面。
The specificity of a horizontally transmitted microbial symbiosis is often defined by molecular communication between host and microbe during initial engagement, which can occur in discrete stages. In the symbiosis between Steinernema nematodes and Xenorhabdus bacteria, previous investigations focused on bacterial colonization of the intestinal lumen (receptacle) of the nematode infective juvenile (IJ), as this was the only known persistent, intimate, and species-specific contact between the two. Here we show that bacteria colonize the anterior intestinal cells of other nematode developmental stages in a species-specific manner. Also, we describe three processes that only occur in juveniles that are destined to become IJs. First, a few bacterial cells colonize the nematode pharyngeal-intestinal valve (PIV) anterior to the intestinal epithelium. Second, the nematode intestine constricts while bacteria initially remain in the PIV. Third, anterior intestinal constriction relaxes and colonizing bacteria occupy the receptacle. At each stage, colonization requires X. nematophila symbiosis region 1 (SR1) genes and is species-specific: X. szentirmaii, which naturally lacks SR1, does not colonize unless SR1 is ectopically expressed. These findings reveal new aspects of Xenorhabdus bacteria interactions with and transmission by their Steinernema nematode hosts, and demonstrate that bacterial SR1 genes aid in colonizing nematode epithelial surfaces.
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影响因子：
2.1
作者：
AKHURST, RJ
通讯作者：
AKHURST, RJ
影响因子：
3.2
作者：
Bhasin, Archna;Chaston, John M.;Goodrich-Blair, Heidi
通讯作者：
Goodrich-Blair, Heidi
影响因子：
3.2
作者：
Gage, DJ
通讯作者：
Gage, DJ
影响因子：
1.3
作者：
Lee, Ming-Min;Stock, S. Patricia
通讯作者：
Stock, S. Patricia
影响因子：
1.2
作者：
Murfin, Kristen E.;Chaston, John;Goodrich-Blair, Heidi
通讯作者：
Goodrich-Blair, Heidi