Auxofuran, a novel metabolite that stimulates the growth of fly agaric, is produced by the mycorrhiza helper bacterium Streptomyces strain AcH 505
Auxofuran, a novel metabolite that stimulates the growth of fly agaric, is produced by the mycorrhiza helper bacterium Streptomyces strain AcH 505
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DOI：
10.1128/aem.72.5.3550-3557.2006
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发表时间：
2006-05-01
影响因子：
4.4
通讯作者：
Fiedler, Hans-Peter
中科院分区：
文献类型：
--
作者：
Riedlinger, Julia;Schrey, Silvia D.;Fiedler, Hans-Peter
菌根辅助菌Streptomyces菌株AcH 505促进了毒伞菌与云杉间外生菌根真菌的菌丝生长和外生菌根的形成，抑制了植物致病性真菌的生长，表明其同时产生了促进真菌生长和抑制真菌生长的化合物。分离得到了菌株ach505的优势促真菌生长物质生长素，并研究了生长素对麻蝇基因表达水平的影响。Auxofuran及其合成类似物7- de羟基Auxofuran在浓度为15 μ M时最有效，并且这些化合物的应用导致脂质代谢相关基因表达增加。菌株AcH 505与蕈状芽孢杆菌共培养可促进链霉菌产生辅酶呋喃。菌株AcH 505产生的抗真菌物质鉴定为抗生素WS-5995 B和c。WS-5995 B在浓度为60 μ M时完全阻断菌丝生长，引起细胞应激相关基因表达应答。这些化合物的鉴定为真菌与云杉外生菌根共生过程中真菌与细菌相互作用的分子分析奠定了基础。
The mycorrhiza helper bacterium Streptomyces strain AcH 505 improves mycelial growth of ectomycorrhizal fungi and formation of ectomycorrhizas between Amanita muscaria and spruce but suppresses the growth of plant-pathogenic fungi, suggesting that it produces both fungal growth-stimulating and -suppressing compounds. The dominant fungal-growth-promoting substance produced by strain AcH 505, auxoforan, was isolated, and its effect on the levels of gene expression of A. muscaria was investigated. Auxofuran and its synthetic analogue 7-dehydroxy-auxofuran were most effective at a concentration of 15 mu M, and application of these compounds led to increased lipid metabolism-related gene expression. Cocultivation of strain AcH 505 and A. muscaria stimulated auxofuran production by the streptomycete. The antifungal substances produced by strain AcH 505 were identified as the antibiotics WS-5995 B and C. WS-5995 B completely blocked mycelial growth at a concentration of 60 mu M and caused a cell stress-related gene expression response in A. muscaria. Characterization of these compounds provides the foundation for molecular analysis of the fungus-bacterium interaction in the ectomycorrhizal symbiosis between fly agaric and spruce.