A high-throughput analysis of high-resolution X-ray CT images of stems of olive and citrus plants resistant and susceptible to Xylella fastidiosa
A high-throughput analysis of high-resolution X-ray CT images of stems of olive and citrus plants resistant and susceptible to Xylella fastidiosa
复制标题
对耐叶缘焦枯病菌和敏感的橄榄和柑橘植物茎的高分辨率 X 射线 CT 图像进行高通量分析
DOI：
10.1111/ppa.13835
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发表时间：
2023
期刊：
影响因子：
2.7
通讯作者：
Walker N
中科院分区：
文献类型：
--
作者：
Walker N
细菌性植物病原体Xylella fastidiosa会导致多种全球重要作物患病。然而，一些品种港口减少细菌负荷和表达很少的症状。考虑隔离植物物种的证据表明木质部结构影响品种对X. fastidiosa的易感性。我们通过分析健康和受感染植物脉管系统的高分辨率同步加速器X射线计算机断层扫描更广泛地测试了这一理论，这些植物脉管系统来自两个分类组，包括敏感和抗性品种：两个柑橘品种（甜橙子cv. Pera，tangor cv. Murcott）和两个橄榄品种（Koroneiki，Leccino）。结果表明，感病植株比抗病植株具有更多的导管，这可以促进病原菌在寄主内的传播。然而，柑橘和橄榄并不具有与抗性相关的特征。虽然抗性柑橘茎中的木质部导管的直径与易感植物中的直径相当，但抗性橄榄的导管较窄，可以限制生物膜的传播。虽然没有检测到橄榄品种之间的差异，结果表明更大的血管连通性，在电阻相比，敏感的柑橘植物。我们假设，这提供了替代的流动路径，以维持感染下的液压功能。总之，这项工作阐明了两个分类组之间不同的生理抗性机制，同时支持存在一个分类间指标，可以加快候选抗性植物的鉴定。
The bacterial plant pathogenXylella fastidiosacauses disease in several globally important crops. However, some cultivars harbour reduced bacterial loads and express few symptoms. Evidence considering plant species in isolation suggests xylem structure influences cultivar susceptibility toX.fastidiosa. We test this theory more broadly by analysing high‐resolution synchrotron X‐ray computed tomography of healthy and infected plant vasculature from two taxonomic groups containing susceptible and resistant varieties: two citrus cultivars (sweet orange cv. Pera, tangor cv. Murcott) and two olive cultivars (Koroneiki, Leccino). Results found the susceptible plants had more vessels than resistant ones, which could promote within‐host pathogen spread. However, features associated with resistance were not shared by citrus and olive. While xylem vessels in resistant citrus stems had comparable diameters to those in susceptible plants, resistant olives had narrower vessels that could limit biofilm spread. And while differences among olive cultivars were not detected, results suggest greater vascular connectivity in resistant compared to susceptible citrus plants. We hypothesize that this provides alternate flow paths for sustaining hydraulic functionality under infection. In summary, this work elucidates different physiological resistance mechanisms between two taxonomic groups, while supporting the existence of an intertaxonomical metric that could speed up the identification of candidate‐resistant plants.