BETA-MOMORCHARIN, A PLANT GLYCOPROTEIN, INHIBITS SYNTHESIS OF MACROMOLECULES IN EMBRYOS, SPLENOCYTES AND TUMOR-CELLS
BETA-MOMORCHARIN, A PLANT GLYCOPROTEIN, INHIBITS SYNTHESIS OF MACROMOLECULES IN EMBRYOS, SPLENOCYTES AND TUMOR-CELLS
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DOI：
10.1016/0020-711x(92)90371-7
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发表时间：
1992-07-01
期刊：
影响因子：
--
通讯作者：
YEUNG, HW
中科院分区：
文献类型：
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作者：
CHAN, WY;NG, TB;YEUNG, HW
1.β-苦瓜素是一种从苦瓜种子中分离出来的糖蛋白，在围着床期小鼠胚胎、伴或不伴刀豆球蛋白A激活的小鼠脾细胞以及人舌和喉鳞状细胞癌中抑制[H-3]亮氨酸、[H-3]尿苷和[H-3]胸苷掺入三氯乙酸可沉淀放射性，但不影响上述放射性同位素掺入小鼠肝细胞。2.结果提示，抑制胚胎、脾细胞和肿瘤细胞蛋白质、RNA和DNA的生物合成可能是β-苦瓜素的胚胎毒性、免疫抑制和抗肿瘤作用的机制。结果还表明，在小鼠肝细胞中，蛋白质、RNA和DNA的生物合成不受β-苦瓜素的影响。
1. Beta-Momorcharin, a glycoprotein isolated from seeds of the bitter gourd, inhibited incorporation of [H-3]leucine, [H-3]uridine and [H-3]thymidine into trichloroacetic acid-precipitable radioactivity in peri-implantation mouse embryos, mouse splenocytes with or without activation by concanavalin A, and human squamous carcinoma of the tongue and larynx, but did not affect incorporation of the aforementioned radioisotopes into mouse liver cells.2. The results suggest that inhibition of protein, RNA and DNA biosynthesis in embryos, splenocytes and tumor cells may represent the mechanism of embryotoxic, immunosuppressive and antitumor actions of beta-momorcharin.3. The results also suggest that in mouse liver cells biosynthesis of protein, RNA and DNA was not affected by beta-momorcharin.