Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.
Increase in Dye:Dendrimer Ratio Decreases Cellular Uptake of Neutral Dendrimers in RAW Cells.
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染料：树枝状聚合物比率的增加会减少原始细胞中中性树枝状聚合物的细胞摄取。
DOI：
10.1021/acsbiomaterials.6b00308
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发表时间：
2016
影响因子：
5.8
通讯作者：
DiMaggio,Stassi
中科院分区：
文献类型：
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作者：
Vaidyanathan,Sriram;Kaushik,Milan;Dougherty,Casey;Rattan,Rahul;Goonewardena,SaschaN;BanaszakHoll,MarkM;Monano,Janet;DiMaggio,Stassi
中性第 3 代聚（酰胺基胺）树枝状聚合物用俄勒冈绿 488 (G3-OGn) 标记，以获得具有受控荧光团：树枝状聚合物比率 (n = 1–2) 的材料、每个树枝状聚合物主要包含 3 种染料的混合物、每个树枝状聚合物主要包含 4 种或更多染料的混合物 (n= 4+) 和随机混合物 (n= 4avg)。染料缀合物的紫外吸光度随着 n 的增加而线性增加，并且荧光发射随着 n 的增加而线性减少。研究了 RAW 细胞和 HEK 293A 细胞的细胞摄取作为荧光团：树枝状聚合物比率 (n) 的函数。 RAW细胞对G3-OGn(n= 3, 4+, 4avg)的细胞摄取显着低于G3-OGn(n= 1, 2)。 HEK 293A 细胞对 G3-OGn(n= 3, 4+, 4avg) 的摄取与 G3-OG1 没有显着差异。因此，观察到荧光团：树枝状聚合物的比例改变了巨噬细胞摄取机制中的摄取程度，但在 HEK 293A 细胞中没有改变。内吞作用的这种差异表明巨噬细胞中存在对颗粒疏水性敏感的途径。
Neutral generation 3 poly(amidoamine) dendrimers were labeled with Oregon Green 488 (G3-OGn) to obtain materials with controlled fluorophore:dendrimer ratios (n = 1–2), a mixture containing mostly 3 dyes per dendrimer, a mixture containing primarily 4 or more dyes per dendrimer (n= 4+), and a stochastic mixture (n= 4avg). The UV absorbance of the dye conjugates increased linearly as n increased and the fluorescence emission decreased linearly as n increased. Cellular uptake was studied in RAW cells and HEK 293A cells as a function of the fluorophore:dendrimer ratio (n). The cellular uptake of G3-OGn(n= 3, 4+, 4avg) into RAW cells was significantly lower than G3-OGn(n= 1, 2). The uptake of G3-OGn(n= 3, 4+, 4avg) into HEK 293A cells was not significantly different from G3-OG1. Thus, the fluorophore:dendrimer ratio was observed to change the extent of uptake in the macrophage uptake mechanism but not in the HEK 293A cell. This difference in endocytosis indicates the presence of a pathway in the macrophage that is sensitive to hydrophobicity of the particle.