The complete sequence and analysis of the large virulence plasmid pSS of Shigella sonnei
The complete sequence and analysis of the large virulence plasmid pSS of Shigella sonnei
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DOI：
10.1016/j.plasmid.2005.03.002
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发表时间：
2005-09-01
期刊：
影响因子：
2.6
通讯作者：
Jin, Q
中科院分区：
文献类型：
--
作者：
Jiang, Y;Yang, F;Jin, Q
测定了宋内志贺菌大毒力质粒pSS的全序列。214-kb质粒由毒力相关基因片段、O-抗原基因簇、一系列复制和维持组成。基因和大量的插入序列（IS）元件。共鉴定出241个开放阅读框（ORF），其中117个与IS元件或转座酶高度同源，57个与已知的发病相关蛋白同源，30个与复制、质粒维持或其他代谢功能相关。37个ORF与已知功能的蛋白质没有相似性，其中两个与任何假设的蛋白质没有显着相似性。有趣的是，在质粒上鉴定了10个编码O-抗原基因簇的ORF，这与大多数其他志贺菌属明显不同。毒性质粒。一种新的毒素-抗毒素系统，一系列的stbDE同源物，被发现在质粒上的复制区的下游，唯一的分离稳定性系统可能是负责pSS的不稳定性。pSS质粒是具有不同来源和功能的基因的混合物。该序列表明，在一系列细菌物种中，IS介导的DNA重组和收购有着非凡的历史。(c)2005年爱思唯尔公司All rights reserved.
The complete sequence of pSS, which is the large virulence plasmid of Shigella sonnei, was determined. The 214-kb plasmid is composed of segments of virulence-associated genes, the O-antigen gene clusters, a range of replication and maintenance. genes, and large numbers of insertion sequence (IS) elements. Two hundred and forty-one open reading frames (ORFs) were identified, of which 117 are highly homologous to IS elements or transposases, 57 are homologous to known pathogenesis-associated proteins, and 30 are related to replication, plasmid maintenance, or other metabolic functions. Thirty-seven ORFs have no similarity to proteins with a known function, including two with no significant similarity to any hypothetical proteins. Interestingly, 10 ORFs encoding O-antigen gene clusters were identified on the plasmid and this is markedly different from most other Shigella spp. virulent plasmids. A novel toxin-antitoxin system, a series of stbDE homologs, was found on the plasmid immediately downstream of the replication region; the sole segregation stability system may be responsible for the instability of pSS. The pSS plasmid is a mixture of genes with different origins and functions. The sequence suggests a remarkable history of IS-mediated recombination and acquisition of DNA across a range of bacterial species. (c) 2005 Elsevier Inc. All rights reserved.