Specific redistribution of cell-penetrating peptides from endosomes to the cytoplasm and nucleus upon laser illumination
Specific redistribution of cell-penetrating peptides from endosomes to the cytoplasm and nucleus upon laser illumination
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DOI：
10.1021/ja044867z
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发表时间：
2004-12-01
影响因子：
15
通讯作者：
Ferrer, M
中科院分区：
文献类型：
--
作者：
Maiolo, JR;Ottinger, EA;Ferrer, M
细胞穿透肽（CPPs）曾被认为是通过非内吞、非能量依赖的过程穿过细胞膜，但后来发现它在活的哺乳动物细胞的囊泡中积累。在这项研究中，我们证明了使用共聚焦显微镜的激光可以使标记的肽偶联CPPs从囊泡重新分布到细胞质和细胞核中。重新分配后，发现细胞具有生物反应性，并且它们在几个小时内保持形态。通过选择性照射其中一个荧光团，可以启动荧光素和alexa633标记的肽的重新分配。这些多肽有可能被用作示踪剂，通过标准荧光共聚焦显微镜的激光照射选择性地将货物生物分子输送到细胞中。
Cell-penetrating peptides (CPPs), once postulated to cross cell membranes in a non-endocytic, non-energy-dependent process, have since been found to accumulate in vesicles in live mammalian cells. In this study, we show that it is possible to use laser light from a confocal microscope to cause labeled peptide-conjugated CPPs to redistribute from vesicles into the cytoplasm and nucleus of cells. Following redistribution, the cells are found to be biologically responsive, and they retain morphology for several hours. It was possible to initiate redistribution of both fluorescein- and Alexa633-labeled peptides by selective irradiation of one of the fluorophores. These peptides could potentially be used as tracers to selectively deliver cargo biomolecules into cells by laser illumination using a standard fluorescence confocal microscope.