Quantification of biofilm accumulation by an optical approach
Quantification of biofilm accumulation by an optical approach
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DOI：
10.1016/s0167-7012(00)00236-0
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发表时间：
2001-02-01
影响因子：
2.2
通讯作者：
Kalvenes, S
中科院分区：
文献类型：
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作者：
Bakke, R;Kommedal, R;Kalvenes, S
基于铜绿假单胞菌实验，评价了用于非侵入性原位测量生物膜光密度和生物膜光学厚度的方法。生物膜光密度（测量为透射通过生物膜的光束的强度降低）与生物膜质量（测量为总碳和细胞质量）相关。该方法比用于确定生物膜质量积累程度的其他常用方法更灵敏且劳动强度更低。通过光学显微镜测量的生物膜光学厚度基于生物膜折射测量被转化为物理厚度。生物膜的折射率接近于水的折射率。研究的铜绿假单胞菌生物膜在不到一周的时间内达到假稳态，具有稳定的液相基质、细胞和TOC浓度以及平均生物膜厚度。然而，由于生物膜密度和粗糙度在3周后仍在增加，因此未达到真正的稳态。(C)2001爱思唯尔科技有限公司。保留所有权利。
Methods for non-invasive, in situ, measurements of biofilm optical density and biofilm optical thickness were evaluated based on Pseudomonas aeruginosa experiments. Biofilm optical density, measured as intensity reduction of a light beam transmitted through the biofilm, correlates with biofilm mass, measured as total carbon and as cell mass. The method is more sensitive and less labor intensive than other commonly used methods for determining extent of biofilm mass accumulation. Biofilm optical thickness, measured by light microscopy, is translated into physical thickness based on biofilm refraction measurements. Biofilm refractive index was found to be close to the refractive index of water. The P. aeruginosa biofilms studied reached a pseudo steady state in less than a week, with stable liquid phase substrate, cell and TOC concentrations and average biofilm thickness. True steady state was, however, not reached as both biofilm density and roughness were still increasing after 3 weeks. (C) 2001 Elsevier Science B.V. All rights reserved.