PDX1 homeobox protein expression in pseudopyloric glands and gastric carcinomas
PDX1 homeobox protein expression in pseudopyloric glands and gastric carcinomas
复制标题
DOI：
10.1136/gut.2003.026609
复制
发表时间：
2004-03-01
期刊：
影响因子：
24.5
通讯作者：
Yuasa, Y
中科院分区：
文献类型：
--
作者：
Sakai, H;Eishi, Y;Yuasa, Y
背景和目标：虽然已经报道了与胃癌发生有关的肠上皮化生是由ParaHox基因CDX 2诱导的，但尚不清楚哪些基因负责假幽门腺的形成以及它们是否在胃癌发生中起作用。胰腺-十二指肠同源框1（PDX 1）也是ParaHox基因，其有助于胰腺、十二指肠和胃窦的发生和发育。方法：采用免疫组化SP法检测95例胃癌组织中PDX 1、粘蛋白MUC 6和MUC 5AC的表达。结果：PDX 1在假幽门腺和肠上皮化生组织中表达较强，在胃癌组织中的表达与胃癌的发生、发展密切相关。在假幽门腺中MUC 6比MUC 5AC更丰富，而在肠上皮化生中MUC 5AC的水平明显高于MUC 6。PDX 1阳性率在分化型癌（39/43，90.7%）和T1期癌（42/43，97.7%）高于未分化型癌（33/52，63.5%）和T2-4期癌（30/52，57.7%）。PDX 1和MUC 6在胃癌组织中呈双阳性表达，且与组织学类型和浸润深度有关。相比之下，PDX 1和MUC 5AC双阳性反应和组织学类型之间没有明显的联系。结论：我们的研究表明，PDX 1在假幽门腺的发展中起着重要的作用，假幽门腺可能反映了与胃癌发生相关的条件。
Background and aims: Although it has been reported that intestinal metaplasia implicated in gastric carcinogenesis is induced by the ParaHox gene CDX2, it is unclear which genes are responsible for the formation of pseudopyloric glands and whether they play a role in gastric carcinogenesis. Pancreatic-duodenal homeobox 1 (PDX1) is also a ParaHox gene which contributes to the genesis and development of the pancreas, duodenum, and antrum. To clarify its significance for the formation of pseudopyloric glands and gastric carcinogenesis, we investigated expression of PDX1 and mucin in gastric carcinomas and surrounding mucosa.Methods: Gastric carcinoma tissues from 95 patients were used for immunohistochemical analyses of PDX1, and mucins MUC6 and MUC5AC.Results: PDX1 was found to be frequently expressed in pseudopyloric glands and intestinal metaplasia. MUC6 was more abundant than MUC5AC in pseudopyloric glands while higher levels of MUC5AC than MUC6 were evident in intestinal metaplasia. The frequency of PDX1 positive reactivity was higher in differentiated type carcinomas (39/43, 90.7%) and T1 carcinomas (42/43, 97.7%) than in undifferentiated type (33/52, 63.5%) and T2-4 (30/52, 57.7%) carcinomas. PDX1 and MUC6 double positive expression was observed in carcinomas, respectively, including the corpus, and also correlated with histological type and depth of invasion. In contrast, no link was apparent between PDX1 and MUC5AC double positive reactivity and histological type.Conclusion: Our study suggests that PDX1 plays an important role in the development of pseudopyloric glands, and that pseudopyloric glands may reflect a condition associated with gastric carcinogenesis.