A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)
A database-driven approach identifies additional diterpene synthase activities in the mint family (Lamiaceae)
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DOI：
10.1074/jbc.ra118.006025
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发表时间：
2019-01-25
影响因子：
4.8
通讯作者：
Hamberger, Bjoern
中科院分区：
文献类型：
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作者：
Johnson, Sean R.;Bhat, Wajid Waheed;Hamberger, Bjoern
唇形科（Lamiaceae）的成员积累了多种工业和药用相关的二萜。我们最近测序叶转录组从48个不同的遗传唇形科物种。在这里，我们总结了现有的化学分类学和酶活性数据的二萜苷酶（diTPS）在唇形科和利用新的转录组，探索diTPS序列和功能空间。选择候选基因的目的是均匀地对序列同源性空间进行采样，并集中在发现diTPS转录物的物种上，但以前没有报道过二萜结构。我们的功能特性的9个II类diTPSs和10个I类diTPSs从11个不同的植物物种，发现5个II类活动，包括两个新的活动，以及一个频谱的I类活动。在II类diTPS中，我们鉴定了来自筋骨草的新-克罗达-4（18），13 E-二烯基二磷酸合酶，其催化多种昆虫拒食剂化合物生物合成中可能的第一步。在I类diTPS中，有一种来自马郁兰（Origanum majorana）的沼泽地二烯合酶，导致在该物种中发现了专门的二萜。我们的研究结果提供了深入的见解，在薄荷科的二萜生物合成的多样化，并建立了一个全面的基础，继续调查的二萜生物合成在唇形科。
Members of the mint family (Lamiaceae) accumulate a wide variety of industrially and medicinally relevant diterpenes. We recently sequenced leaf transcriptomes from 48 phylogenetically diverse Lamiaceae species. Here, we summarize the available chemotaxonomic and enzyme activity data for diterpene synthases (diTPSs) in the Lamiaceae and leverage the new transcriptomes to explore the diTPS sequence and functional space. Candidate genes were selected with an intent to evenly sample the sequence homology space and to focus on species in which diTPS transcripts were found, yet from which no diterpene structures have been previously reported. We functionally characterized nine class II diTPSs and 10 class I diTPSs from 11 distinct plant species and found five class II activities, including two novel activities, as well as a spectrum of class I activities. Among the class II diTPSs, we identified a neo-cleroda-4(18),13E-dienyl diphosphate synthase from Ajuga reptans, catalyzing the likely first step in the biosynthesis of a variety of insect-antifeedant compounds. Among the class I diTPSs was a palustradiene synthase from Origanum majorana, leading to the discovery of specialized diterpenes in that species. Our results provide insights into the diversification of diterpene biosynthesis in the mint family and establish a comprehensive foundation for continued investigation of diterpene biosynthesis in the Lamiaceae.