Novel blaROB-1-Bearing Plasmid Conferring Resistance to β-Lactams in Haemophilus parasuis Isolates from Healthy Weaning Pigs
Novel blaROB-1-Bearing Plasmid Conferring Resistance to β-Lactams in Haemophilus parasuis Isolates from Healthy Weaning Pigs
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DOI：
10.1128/aem.03865-14
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Garmendia, Junkal
中科院分区：
文献类型：
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作者：
Moleres, Javier;Santos-Lopez, Alfonso;Garmendia, Junkal
副猪嗜血杆菌是格拉瑟氏病的病原体，是仔猪鼻粘膜的早期定植者之一。它在猪群中流行，与疾病相关的病变是纤维蛋白性多浆膜炎和支气管肺炎。抗生素常用于疾病控制，副猪嗜血菌对多种抗生素具有耐药性。目前，由于我们对其致病性知之甚少，副猪嗜血菌毒力的预测受到限制。一些基因与副猪嗜血菌毒力相关，例如 lsgB 和第 1 组 vtaA，而生物膜生长与非毒力菌株相关。在这项研究中，通过对断奶仔猪进行采样，从 10 多年没有出现过该病病例的农场中获得了 86 份副猪嗜血菌鼻分离株。通过肠杆菌重复基因间一致性 PCR 来研究分离株，并测定 lsgB 和第 1 组 vtaA 的存在、生物膜形成、炎症细胞反应和抗生素耐药性。作为所遇到的多样性的一部分，在八个定植菌株中检测到了一种新的 2,661 bp 质粒，名为 pJMA-1，带有 bla(ROB-1) β-内酰胺酶。 pJMA-1 与巴斯德氏菌科中描述的其他小质粒共享主链，100% 稳定，并且比之前描述的质粒 pB1000 具有更低的生物成本。 pJMA-1 也在来自单独收集的 9 种副猪嗜血杆菌鼻菌株中发现，但在患有格拉瑟病的动物病变分离株或无法分型的流感嗜血杆菌分离株中未检测到 pJMA-1。总而言之，我们表明共生副猪嗜血菌分离株代表了β-内酰胺抗性基因的库，这些基因可以转移到病原体或其他细菌中。
Haemophilus parasuis, the causative agent of Glasser's disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the bla(ROB-1) beta-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glasser's disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of beta-lactam resistance genes which can be transferred to pathogens or other bacteria.