Comparison of Campylobacter jejuni lipooligosaccharide biosynthesis loci from a variety of sources
Comparison of Campylobacter jejuni lipooligosaccharide biosynthesis loci from a variety of sources
复制标题
DOI：
10.1128/jcm.43.6.2771-2781.2005
复制
发表时间：
2005-06-01
影响因子：
9.4
通讯作者：
Mandrell, RE
中科院分区：
文献类型：
--
作者：
Parker, CT;Horn, ST;Mandrell, RE
空肠弯曲杆菌菌株在低脂寡糖（LOS）生物合成位点的遗传含量上存在显著差异，同时LOS的结构也存在差异。根据基因含量和组织结构，将空肠C. LOS基因座分为6类。利用这些基因座的PCR扩增，我们能够从123株空肠梭菌（包括39株Penner血清型参考菌株）中对大多数（80%）LOS生物合成基因座进行分类。我们发现，特定的LOS类型并不总是与特定的Penner血清型相关，在本研究中测试的16株格林-巴利综合征相关分离株中有14株具有相同的LOS类型。根据基因特异性PCR的结果和长（XL） PCR测定的LOS生物合成位点的长度，通常可以区分其余无法分类的分离株。其中两个独特的XL PCR产物的序列分析显示了两个新的LOS类。这些结果支持了LOS位点是基因交换和重排热点的假设。利用PCR方法分析LOS生物合成基因可用于空肠梭菌的分型，并为推断潜在的LOS结构提供了有利条件。
Campylobacter jejuni strains exhibit significant variation in the genetic content of the lipooligosaccharide (LOS) biosynthesis loci with concomitant differences in LOS structure. The C. jejuni LOS loci have been grouped into six classes based on gene content and organization. Utilizing PCR amplifications of genes from these loci, we were able to classify a majority (80%) of the LOS biosynthesis loci from 123 strains of C. jejuni that included 39 of the Penner serotype reference strains. We found that a particular LOS class was not always associated with a specific Penner serotype, and 14 of 16 Guillain-Barre syndrome-associated isolates tested in this study shared the same LOS class. The remaining isolates that could not be classified were often distinguishable from each other based on the results of gene-specific PCR and the lengths of their LOS biosynthesis loci as determined by long (XL) PCR. Sequence analysis of two of these unique XL PCR products demonstrated two new LOS classes. These results support the hypothesis that the LOS locus is a hot spot for genetic exchange and rearrangements. Analysis of the LOS biosynthesis genes by PCR assays can be used for typing C. jejuni and offers the advantage of inferring potential LOS structures.