MITOCHONDRIAL FUNCTIONS MEDIATE CELLULASE GENE-EXPRESSION IN TRICHODERMA-REESEI
MITOCHONDRIAL FUNCTIONS MEDIATE CELLULASE GENE-EXPRESSION IN TRICHODERMA-REESEI
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DOI：
10.1021/bi00033a018
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发表时间：
1995-08-22
期刊：
影响因子：
2.9
通讯作者：
ELDORRY, H
中科院分区：
文献类型：
--
作者：
ABRAHAONETO, J;ROSSINI, CHB;ELDORRY, H
我们研究了抑制线粒体功能对编码丝状真菌里氏木霉纤维素酶系统的胞外纤维二糖水解酶 I (cbh1) 和内切葡聚糖酶 I (egll) 的两个核基因表达的影响。 cbhl 和 egll 转录物在低氧张力下受到抑制，并且在已知抑制线粒体呼吸的浓度下受到葡萄糖的抑制。已知可消除线粒体内膜质子电化学梯度并阻断电子传递链的化学试剂（例如 DNP 和 KCN）也会下调转录本。这些结果表明这些转录物的表达受到线粒体生理状态的影响。此外，异源基因融合表明，这些转录物的表达对线粒体功能状态的敏感性是通过这些基因的 5' 侧翼 DNA 序列进行转录控制的。
We examined the effects of inhibition of mitochondrial functions on the expression of two nuclear genes encoding the extracellular cellobiohydrolase I (cbh1) and endoglucanase I (egll) of the cellulase system of the filamentous fungus Trichoderma reesei. The cbhl and egll transcripts are repressed at a low oxygen tension, and by glucose at a concentration known to repress mitochondrial respiration. The transcripts are also down-regulated by chemical agents known to dissipate the proton electrochemical gradient of the inner mitochondrial membrane and blocking of the electron-transport chain, such as DNP and KCN, respectively. These results suggest that expression of those transcripts is influenced by the physiological state of the mitochondria. In addition, heterologous gene fusion shows that the sensitivity of the expression of those transcripts to the functional state of the mitochondria is transcriptionally controlled through the 5'-flanking DNA sequence of those genes.