Mitochondrial genetic markers for authentication of major Red Sea grouper species (Perciformes: Serranidae) in Egypt: A tool for enhancing fisheries management and species conservation
Mitochondrial genetic markers for authentication of major Red Sea grouper species (Perciformes: Serranidae) in Egypt: A tool for enhancing fisheries management and species conservation
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DOI：
10.1016/j.gene.2018.12.021
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发表时间：
2019-03-20
期刊：
影响因子：
3.5
通讯作者：
Mohammed-Geba, Khaled
中科院分区：
文献类型：
--
作者：
Galal-Khallaf, Asmaa;Osman, Alaa G. M.;Mohammed-Geba, Khaled
石斑鱼是具重要生态及经济价值的珊瑚鱼种。它们与其他珊瑚礁生物之间的相互作用有助于这一基本海洋生态位的健康和物种平衡。此外，石斑鱼是价格最高的渔业物种之一。红海的埃及生境缺乏遗传研究，评估物种多样性，以实现养护和渔业管理的最终目标。此外，这些生物体之间的形态相似性有时会妨碍正确的物种鉴定。因此，更准确的石斑鱼身份验证方法是至关重要的。对埃及红海的石斑鱼属Epinealphus、Anyperodon、Cephaolophole、Aethaloperca、Variola和Plectropomus等16种石斑鱼进行了线粒体DNA变异、细胞色素氧化酶亚基I（COI）和12srRNA基因测序。进行了GenBank比对、系统发育分析和两两距离比较。所有这些分析都是为了在国际范围内进行物种鉴定和确定它们之间的关系。与E. fasciatus，A. rogaa角oligosticta、寡点艾美球虫E. areolatus、V.loud、P.areolatus、E. malabaricus、C. sexmaculata、E. summana，E. chlorostigma，E. polyphekadion，C. miniataus、尖吻金丝雀A. leucogrammicus、E. taught，C. argus，C.半球形。从种间到种属间的比较水平提高后，两两距离明显增加。结合12srRNA和COI基因测序导致埃及红海石斑鱼物种明确的歧视的准确工具。这可以为埃及和世界上这些物种的保护和渔业管理提供重要的帮助。
Groupers are coral fish species of prime ecological and economic significance. The interactions among them and other coral reefs organisms aid the healthiness and species balance in this fundamental marine niches. Also, groupers are among the top priced fisheries species. The Egyptian habitats of the Red Sea are lacking genetic studies that assess species diversity for the final goal of conservation and fisheries management. Moreover, morphological similarities among these organisms sometimes hinder a proper species identification. Hence, more accurate groupers authentication methods are crucially required. Sixteen grouper species belonging to the genera Epinephelus, Anyperodon, Cephaolopholes, Aethaloperca, Variola, and Plectropomus, present in the Red Sea in Egypt, were investigated for species authentication through mitochondrial DNA variations, applying cytochrome oxidase subunit I (COI) and 12srRNA genes sequencing. GenBank comparisons, phylogenetic analyses and comparisons of pairwise distances were carried out. All these analyses aimed to species authentication and identifying their relations at the international scale. The results exhibited > 98% identity with E. fasciatus, A. rogaa, C. oligosticta, E. areolatus, V. loud, P. areolatus, E. malabaricus, C. sexmaculata, E. summana, E. chlorostigma, E. polyphekadion, C. miniataus, A. leucogrammicus, E. tauvina, C. argus, C. hemistiktos. Pairwise distances showed a clear increase upon raising comparison level from among species to among-genera. Combined 12srRNA and COI genes sequencing resulted in an accurate tool for Egyptian Red Sea grouper species unambiguous discrimination. This can provide vital aid to the active efforts for these species conservation and fisheries management in Egypt and the world.